PF 06465469

Pricing Availability   Qty
Description: Potent ITK inhibitor; also inhibits BTK
Chemical Name: (R)-3-(1-(1-Acryloylpiperidin-3-yl)-4-amino-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-N-(3-methyl-4-(1-methylethyl))benzamide
Purity: ≥95% (HPLC)
Citations (2)

Biological Activity for PF 06465469

PF 06465469 is a potent inhibitor of interleukin-2 inducible T cell kinase (ITK) (IC50 = 2 nM). Also exhibits inhibitory activity against Bruton's tyrosine kinase (BTK) (IC50 = 2 nM). Displays nanomolar potencies in a cell-based IP1 assay and human whole blood assay (IC50 values are 31 and 48 nM respectively).

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Technical Data for PF 06465469

M. Wt 523.63
Formula C30H33N7O2
Storage Store at +4°C
Purity ≥95% (HPLC)
CAS Number 1407966-77-1
PubChem ID 71450146
Smiles NC1=C2C(N([C@@H]4CCCN(C(C=C)=O)C4)N=C2C3=CC(C(NC5=CC(C)=C(C(C)C)C=C5)=O)=CC=C3)=NC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PF 06465469

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 2.62 5 with gentle warming

Preparing Stock Solutions for PF 06465469

The following data is based on the product molecular weight 523.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 38.19 mL 190.97 mL 381.95 mL
0.25 mM 7.64 mL 38.19 mL 76.39 mL
0.5 mM 3.82 mL 19.1 mL 38.19 mL
2.5 mM 0.76 mL 3.82 mL 7.64 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for PF 06465469

References are publications that support the biological activity of the product.

Zapf et al (2012) Covalent inhibitors of interleukin-2 inducible T cell kinase (Itk) with nanomolar potency in a whole-blood assay. J.Med.Chem. 55 10047 PMID: 23098091

If you know of a relevant reference for PF 06465469, please let us know.

View Related Products by Target

View Related Products by Product Action

View all ITK Inhibitors

Keywords: PF 06465469, PF 06465469 supplier, PF06465469, Pfizer, interleukin-2, IL-2, inducible, T, cell, kinase, ITK, inhibitors, inhibits, potent, Brutons, tyrosine, BTK, Tyrosine, Kinase, (BTK), 4710, Tocris Bioscience

2 Citations for PF 06465469

Citations are publications that use Tocris products. Selected citations for PF 06465469 include:

Mamand et al (2018) Comparison of interleukin-2-inducible kinase (ITK) inhibitors and potential for combination therapies for T-cell lymphoma. Sci Rep 8 14216 PMID: 30242208

Christopher J et al (2022) Pharmacologic Targeting of TFIIH Suppresses KRAS-Mutant Pancreatic Ductal Adenocarcinoma and Synergizes with TRAIL. Cancer Res 82 3375-3393 PMID: 35819261

Do you know of a great paper that uses PF 06465469 from Tocris? Please let us know.

Reviews for PF 06465469

There are currently no reviews for this product. Be the first to review PF 06465469 and earn rewards!

Have you used PF 06465469?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review