PF 06409577

Pricing Availability   Qty

Save up to 40% on RUO Reagents with BIOSPRING24 (see details)

Description: Potent and selective allosteric activator of AMPK
Chemical Name: 6-Chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylic acid
Purity: ≥98% (HPLC)
Citations (1)
Pathways (1)

Biological Activity for PF 06409577

PF 06409577 is a potent and selective allosteric activator of AMPK (EC50 values are 7 and >40000 nM for AMPKα1β1γ1 and AMPKα1β2γ1, respectively). Exhibits minimal off-target effects on a panel of receptors, ion channels, PDEs and kinases. Improves kidney function and reduces proteinuria in a preclinical model of diabetic nephropathy. Orally bioavailable.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Compound Libraries for PF 06409577

PF 06409577 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for PF 06409577

M. Wt 341.79
Formula C19H16ClNO3
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 1467057-23-3
PubChem ID 71748255
Smiles O=C(O)C1=CNC2=CC(Cl)=C(C3=CC=C(C4(CCC4)O)C=C3)C=C21

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PF 06409577

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 34.18 100
ethanol 6.84 20

Preparing Stock Solutions for PF 06409577

The following data is based on the product molecular weight 341.79. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.93 mL 14.63 mL 29.26 mL
5 mM 0.59 mL 2.93 mL 5.85 mL
10 mM 0.29 mL 1.46 mL 2.93 mL
50 mM 0.06 mL 0.29 mL 0.59 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for PF 06409577

Certificate of Analysis / Product Datasheet
Select another batch:

References for PF 06409577

References are publications that support the biological activity of the product.

Cameron et al (2016) Discovery and preclinical characterization of 6-Chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylic Acid (PF-06409577), a direct activator of adenosine monophosphate-activated protein kinase (AMPK), for the potential treatment of diab J.Med.Chem. 59 8068 PMID: 27490827

Salatto et al (2017) Selective activation of AMPK β1-containing isoforms improves kidney function in a rat model of diabetic nephropathy. J.Pharmacol.Exp.Ther. 361 303 PMID: 28289077

If you know of a relevant reference for PF 06409577, please let us know.

View Related Products by Target

View Related Products by Product Action

View all AMPK Activators

Keywords: PF 06409577, PF 06409577 supplier, PF06409577, AMP-activated, protein, kinase, AMPK, activators, activates, diabetic, nephropathy, potent, selective, allosteric, orally, bioavailable, α1β1γ1, Enzyme, Substrates, /, Activators, 6114, Tocris Bioscience

1 Citation for PF 06409577

Citations are publications that use Tocris products. Selected citations for PF 06409577 include:

Smith et al (2019) An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis. Nat Commun 10 2901 PMID: 31263101

Do you know of a great paper that uses PF 06409577 from Tocris? Please let us know.

Reviews for PF 06409577

There are currently no reviews for this product. Be the first to review PF 06409577 and earn rewards!

Have you used PF 06409577?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Pathways for PF 06409577

AMPK Signaling Pathway

AMPK Signaling Pathway

AMPK signaling pathway plays an important role in the cellular reponse to low levels of available ATP, often caused by stresses such as heat shock, ischemia or hypoxia.