PF 04449613

Pricing Availability   Qty

Save up to 40% on RUO Reagents with BIOSPRING24 (see details)

Description: Potent PDE9 inhibitor; brain penetrant
Alternative Names: PF 9613
Chemical Name: 1,5-Dihydro-6-[(1R)-1-(3-phenoxy-1-azetidinyl)ethyl]-1-(tetrahydro-2H-pyran-4-yl)-4H-pyrazolo[3,4-d]pyrimidin-4-one
Purity: ≥98% (HPLC)

Biological Activity for PF 04449613

PF 04449613 is a potent PDE9 inhibitor (IC50 = 22 nM). PF 4449613 shows more than 1000-fold selectivity for PDE9A over most of 79 other non-PDE targets investigated, except for cytochrome P450 2C19 (IC50 = 1600 nM), dopamine transporter (Ki = 110 nM), μ-opioid receptor (Ki =3500 nM), and sodium channel binding site 2 (Ki =470 nM). PF 04449613 reduces body fat in mice with diet-induced obesity, stimulating mitochondrial activity in brown and white fat, and improving cardiometabolic syndrome symptoms. Brain penetrant. PF 04449613 increases synaptic calcium activity and learning-dependent synaptic plasticity in mice.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Compound Libraries for PF 04449613

PF 04449613 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for PF 04449613

M. Wt 395.45
Formula C21H25N5O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1236858-52-8
PubChem ID 46851937
Smiles C[C@H](C1=NC(C2=C(N(C3CCOCC3)N=C2)N1)=O)N4CC(OC5=CC=CC=C5)C4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PF 04449613

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 39.55 100

Preparing Stock Solutions for PF 04449613

The following data is based on the product molecular weight 395.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.53 mL 12.64 mL 25.29 mL
5 mM 0.51 mL 2.53 mL 5.06 mL
10 mM 0.25 mL 1.26 mL 2.53 mL
50 mM 0.05 mL 0.25 mL 0.51 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for PF 04449613

Certificate of Analysis / Product Datasheet
Select another batch:

References for PF 04449613

References are publications that support the biological activity of the product.

Kleiman et al (2012) Phosphodiesterase 9A regulates central cGMP and modulates responses to cholinergic and monoaminergic perturbation in vivo. J.Pharmacol.Exp.Ther. 341 396 PMID: 22328573

Lee et al (2015) Phosphodiesterase 9A controls nitric-oxide-independent cGMP and hypertrophic heart disease. Nature 519 472 PMID: 25799991

Claffey et al (2012) Application of structure-based drug design and parallel chemistry to identify selective, brain penetrant, in vivo active phosphodiesterase 9A inhibitors. J.Med.Chem. 55 9055 PMID: 23025719

Mishra et al (2021) Inhibition of phosphodiesterase type 9 reduces obesity and cardiometabolic syndrome in mice. J.Clin.Invest. 131 e148798 PMID: 34618683

Lai et al (2018) The phosphodiesterase 9 inhibitor PF-04449613 promotes dendritic spine formation and performance improvement after motor learning. Dev.Neurobiol. 78 859 PMID: 30022611

If you know of a relevant reference for PF 04449613, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Phosphodiesterase Inhibitors

Keywords: PF 04449613, PF 04449613 supplier, PF04449613, PF9613, phosphodiesterases, PDE, A, inhibitors, inhibits, potent, Schizophrenia, Alzheimer's, disease, brain, penetrant, PF, 9613, Phosphodiesterases, 5915, Tocris Bioscience

Citations for PF 04449613

Citations are publications that use Tocris products.

Currently there are no citations for PF 04449613. Do you know of a great paper that uses PF 04449613 from Tocris? Please let us know.

Reviews for PF 04449613

There are currently no reviews for this product. Be the first to review PF 04449613 and earn rewards!

Have you used PF 04449613?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review