Cyclin-dependent kinase inhibitor that competes for the ATP binding site of the kinase. Selectively inhibits cdc2/cyclin B (IC50 = 7 μM), cdk2/cyclin A (IC50 = 7 μM), cdk2/cyclin E (IC50 = 7 μM), cdk/p35 kinase (IC50 = 3 μM) and ERK1/MAP kinase (IC50 = 25 μM). Arrests human fibroblasts in the G1 phase.
|Storage||Desiccate at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solubility||Soluble to 50 mM in ethanol|
Preparing Stock Solutions
The following data is based on the product molecular weight 298.35. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.35 mL||16.76 mL||33.52 mL|
|5 mM||0.67 mL||3.35 mL||6.7 mL|
|10 mM||0.34 mL||1.68 mL||3.35 mL|
|50 mM||0.07 mL||0.34 mL||0.67 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Abraham et al (1995) Cellular effects of olomoucine, an inhibitor of cyclin-dependent kinases. Biol.Cell 83 105 PMID: 7549905
Alessi et al (1998) The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity. Exp.Cell.Res. 245 8 PMID: 9828096
Vesely et al (1994) Inhibition of cyclin-dependent kinases by purine analogues. Eur.J.Biochem. 224 771 PMID: 7925396
If you know of a relevant reference for Olomoucine, please let us know.
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Keywords: Olomoucine, supplier, Cyclin-dependent, protein, kinases, inhibitors, inhibits, Cdks, Cyclin-dependent, Kinase, Tocris Bioscience
1 Citation for Olomoucine
Citations are publications that use Tocris products. Selected citations for Olomoucine include:
Cannon et al (2009) Association of luteinizing hormone receptor gene expression with cell cycle progression in granulosa cells. Am J Physiol Endocrinol Metab 296 E1392 PMID: 19293332
Do you know of a great paper that uses Olomoucine from Tocris? If so please let us know.
Literature in this Area
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In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.