OGDA

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Cat.No. 7408 - OGDA | C24H16F2N2O8
Description: Green fluorescent D-amino acid; compatible with STED microscopy
Alternative Names: OG fluorescent D-amino acid, OG FDAA
Chemical Name: (R)-2-Amino-3-(2',7'-difluoro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxamido)propanoic acid
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for OGDA

OGDA is a green fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. OGDA is efficiently incorporated into the peptidoglycans of gram-positive and some gram-negative bacteria. Compatible with STED microscopy, OGDA labelled bacteria can be imaged by super-resolution stimulated emission depletion (STED) at a resolution below 100 nm. Photo-stable. Excitation/emission λ ~501/526 nm.

Optical Data for OGDA

OGDA Dye Spectra
Emission Color Green
λabs 501 nm
λem 526 nm
Closest Laser line 488 nm
Application STED, Super resolution microscopy, Confocal microscopy

Technical Data for OGDA

M. Wt 498.39
Formula C24H16F2N2O8
Storage Store at -20°C
Purity ≥98% (HPLC)
InChI Key RFRUFDKIAPOSBS-MRXNPFEDSA-N
Smiles O=C(NC[C@H](C(O)=O)N)C1=CC=C(C2=C1)C(OC32C4=C(OC5=C3C=C(C(O)=C5)F)C=C(O)C(F)=C4)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for OGDA

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 49.84 100

Preparing Stock Solutions for OGDA

The following data is based on the product molecular weight 498.39. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.01 mL 10.03 mL 20.06 mL
5 mM 0.4 mL 2.01 mL 4.01 mL
10 mM 0.2 mL 1 mL 2.01 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References for OGDA

References are publications that support the biological activity of the product.

Söderström et al (2020) An OregonGreen488-labelled D-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy. Microbiology 166 1129 PMID: 33237852


If you know of a relevant reference for OGDA, please let us know.

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Citations for OGDA

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Literature in this Area

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