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Description: MDM2 antagonist; inhibits MDM2-p53 interaction
Chemical Name: (±)-4-[4,5-Bis(4-chlorophenyl)-2-(2-isopropoxy-4-methoxy-phenyl)-4,5-dihydro-imidazole-1-carbonyl]-piperazin-2-one
Purity: ≥98% (HPLC)
Citations (8)
Reviews (1)
Literature (3)

Biological Activity for Nutlin-3

Nutlin-3 is a MDM2 antagonist; inhibits the MDM2-p53 interaction (IC50 = 0.09 μM) and activates p53. Antiproliferative agent; induces apoptosis in cancer cells.

Licensing Information

Sold under license from F. Hoffmann-La Roche Ltd.

Technical Data for Nutlin-3

M. Wt 581.49
Formula C30H30Cl2N4O4
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 548472-68-0
PubChem ID 16755649
Smiles ClC1=CC=[C@@]([C@@H]3[C@@H](N(C(N5CC(NCC5)=O)=O)C(C4=CC=C(OC)C=C4OC(C)C)=N3)[C@]2=CC=C(Cl)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Nutlin-3

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 58.15 100
ethanol 58.15 100

Preparing Stock Solutions for Nutlin-3

The following data is based on the product molecular weight 581.49. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.72 mL 8.6 mL 17.2 mL
5 mM 0.34 mL 1.72 mL 3.44 mL
10 mM 0.17 mL 0.86 mL 1.72 mL
50 mM 0.03 mL 0.17 mL 0.34 mL

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Product Datasheets for Nutlin-3

Certificate of Analysis / Product Datasheet
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References for Nutlin-3

References are publications that support the biological activity of the product.

Vassilev et al (2004) In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science 303 844 PMID: 14704432

If you know of a relevant reference for Nutlin-3, please let us know.

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Keywords: Nutlin-3, Nutlin-3 supplier, Nutlin3, p53, activator, MDM2, antagonist, antagonists, p53-MDM2, Ubiquitin, E3, Ligases, 3984, Tocris Bioscience

8 Citations for Nutlin-3

Citations are publications that use Tocris products. Selected citations for Nutlin-3 include:

Daniel P et al (2021) Replication stress promotes cell elimination by extrusion. Nature 593 591-596 PMID: 33953402

Bussey et al (2016) Targeting polo-like kinase 1, a regulator of p53, in the treatment of adrenocortical carcinoma. J Zhejiang Univ Sci B 5 1 PMID: 26754547

Gergely et al (2022) Comparison of Different Clinical Chemotherapeutical Agents' Toxicity and Cell Response on Mesenchymal Stem Cells and Cancer Cells. Cells 11 PMID: 36230904

Alan R et al (2013) Small molecule induced reactivation of mutant p53 in cancer cells. Nucleic Acids Res 41 6034-44 PMID: 23630318

Do you know of a great paper that uses Nutlin-3 from Tocris? Please let us know.

Reviews for Nutlin-3

Average Rating: 5 (Based on 1 Review.)

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In vitro cell treatment using Nutlin (Cat. No. 3984).
By Anonymous on 01/08/2018
Assay Type: In Vitro
Species: Human

It worked perfectly at 10microM concentration for 48 hours.

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Targeted Protein Degradation Research Product Guide

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

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There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

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Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia