Nutlin-3

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Cat.No. 3984 - Nutlin-3 | C30H30Cl2N4O4 | CAS No. 548472-68-0
Description: MDM2 antagonist; inhibits MDM2-p53 interaction
Chemical Name: (±)-4-[4,5-Bis(4-chlorophenyl)-2-(2-isopropoxy-4-methoxy-phenyl)-4,5-dihydro-imidazole-1-carbonyl]-piperazin-2-one
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Literature

Biological Activity

MDM2 antagonist; inhibits the MDM2-p53 interaction (IC50 = 0.09 μM) and activates p53. Antiproliferative agent; induces apoptosis in cancer cells.

Licensing Information

Sold under license from F. Hoffmann-La Roche Ltd.

Compound Libraries

Nutlin-3 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 581.49
Formula C30H30Cl2N4O4
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 548472-68-0
PubChem ID 16755649
InChI Key BDUHCSBCVGXTJM-IZLXSDGUSA-N
Smiles ClC1=CC=[C@@]([C@@H]3[C@@H](N(C(N5CC(NCC5)=O)=O)C(C4=CC=C(OC)C=C4OC(C)C)=N3)[C@]2=CC=C(Cl)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 58.15 100
ethanol 58.15 100

Preparing Stock Solutions

The following data is based on the product molecular weight 581.49. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.72 mL 8.6 mL 17.2 mL
5 mM 0.34 mL 1.72 mL 3.44 mL
10 mM 0.17 mL 0.86 mL 1.72 mL
50 mM 0.03 mL 0.17 mL 0.34 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Vassilev et al (2004) In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science 303 844 PMID: 14704432


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1 Citation for Nutlin-3

Citations are publications that use Tocris products. Selected citations for Nutlin-3 include:

Bussey et al (2016) Targeting polo-like kinase 1, a regulator of p53, in the treatment of adrenocortical carcinoma. J Zhejiang Univ Sci B 5 1 PMID: 26754547


Do you know of a great paper that uses Nutlin-3 from Tocris? If so please let us know.

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Literature in this Area

Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Nutlin-3

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