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Microtubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 301.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.5 mM||6.64 mL||33.19 mL||66.37 mL|
|2.5 mM||1.33 mL||6.64 mL||13.27 mL|
|5 mM||0.66 mL||3.32 mL||6.64 mL|
|25 mM||0.13 mL||0.66 mL||1.33 mL|
References are publications that support the biological activity of the product.
Vasquez et al (1997) Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 8 973 PMID: 9201709
Webb et al (2004) Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 36 2541 PMID: 15325591
Mizushima et al (2010) Methods in mammalian autophagy research. Cell 140 313 PMID: 20144757
Lin et al (2014) Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 3 e04766 PMID: 25497837
If you know of a relevant reference for Nocodazole, please let us know.
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Keywords: Nocodazole, Nocodazole supplier, Microtubule, inhibitors, inhibits, Tau, Tubulin, Microtubules, Mitosis, crispr, cas9, homology-directed, repair, HDR, Autophagy, CRISPR, Reagents, 1228, Tocris Bioscience
12 Citations for Nocodazole
Citations are publications that use Tocris products. Selected citations for Nocodazole include:
Macheret and Halazonetis (2018) Intragenic origins due to short G1 phases underlie oncogene-induced DNA replication stress. Nature 555 112 PMID: 29466339
Romero et al (2018) Damage-induced reactive oxygen species enable zebrafish tail regeneration by repositioning of Hedgehog expressing cells. Nat Commun 9 4010 PMID: 30275454
Schapitz et al (2010) Neuroligin 1 is dynamically exchanged at postsynaptic sites. Oncotarget 30 12733 PMID: 20861378
Lin et al (2015) Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Nat Commun 6 18577 PMID: 26124177
Tauchman et al (2015) Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Cell Biol 6 10036 PMID: 26620470
Seese et al (2012) LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice. J Neurosci 32 7403 PMID: 22623686
Bernier et al (2019) Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep 27 2895 PMID: 31167136
Melemedjian et al (2014) Local translation and retrograde axonal transport of CREB regulates IL-6-induced nociceptive plasticity. Mol Pain 10 45 PMID: 24993495
Paonessa et al (2019) Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia. Cell Rep 26 582 PMID: 30650353
Guo et al (2016) Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Front Cell Neurosci 18 202 PMID: 26655835
Hutchins and Wray (2014) Capture of microtubule plus-ends at the actin cortex promotes axophilic neuronal migration by enhancing microtubule tension in the leading process. Methods Mol Biol 8 400 PMID: 25505874
Rood et al (2011) Methods for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose). Anal Biochem 780 153 PMID: 21870260
Do you know of a great paper that uses Nocodazole from Tocris? Please let us know.
Reviews for Nocodazole
Average Rating: 5 (Based on 1 Review.)
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Used in brain slcies to disturb microtubule dynamics. Effect on cell shape was observed within minutes
Literature in this Area
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