Nocodazole

Pricing Availability   Qty
Cat.No. 1228 - Nocodazole | C14H11N3O3S | CAS No. 31430-18-9
Description: Microtubule inhibitor
Chemical Name: [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]carbonic acid, methyl ester
Purity: ≥95% (HPLC)
Datasheet
Citations (12)
Reviews (1)
Literature (2)

Biological Activity

Microtubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.

Technical Data

M. Wt 301.32
Formula C14H11N3O3S
Storage Store at RT
Purity ≥95% (HPLC)
CAS Number 31430-18-9
PubChem ID 4122
InChI Key KYRVNWMVYQXFEU-UHFFFAOYSA-N
Smiles COC(=O)NC1=NC2=CC(=CC=C2N1)C(=O)C1=CC=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 15.07 50

Preparing Stock Solutions

The following data is based on the product molecular weight 301.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 6.64 mL 33.19 mL 66.37 mL
2.5 mM 1.33 mL 6.64 mL 13.27 mL
5 mM 0.66 mL 3.32 mL 6.64 mL
25 mM 0.13 mL 0.66 mL 1.33 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:

References

References are publications that support the biological activity of the product.

Vasquez et al (1997) Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 8 973 PMID: 9201709

Webb et al (2004) Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 36 2541 PMID: 15325591

Mizushima et al (2010) Methods in mammalian autophagy research. Cell 140 313 PMID: 20144757

Lin et al (2014) Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 3 e04766 PMID: 25497837


If you know of a relevant reference for Nocodazole, please let us know.

Keywords: Nocodazole, Nocodazole supplier, Microtubule, inhibitors, inhibits, Tau, Tubulin, Microtubules, Mitosis, crispr, cas9, homology-directed, repair, HDR, Autophagy, CRISPR, Reagents, 1228, Tocris Bioscience

12 Citations for Nocodazole

Citations are publications that use Tocris products. Selected citations for Nocodazole include:

Bernier et al (2019) Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep 27 2895 PMID: 31167136

Paonessa et al (2019) Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia. Cell Rep 26 582 PMID: 30650353

Guo et al (2016) Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Front Cell Neurosci 18 202 PMID: 26655835

Rood et al (2011) Methods for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose). Anal Biochem 780 153 PMID: 21870260


Do you know of a great paper that uses Nocodazole from Tocris? Please let us know.

Reviews for Nocodazole

Average Rating: 5 (Based on 1 Review.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Nocodazole?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Microtubule disruption.
By Anonymous on 01/30/2018
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: Brain slice

Used in brain slcies to disturb microtubule dynamics. Effect on cell shape was observed within minutes


Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Neurodegeneration

Neurodegeneration Product Guide

A collection of over 275 products for neurodegeneration research, the guide includes research tools for the study of:

  • Alzheimer's disease
  • Parkinson's disease
  • Huntington's disease