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Description: Microtubule inhibitor
Chemical Name: [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]carbonic acid, methyl ester
Purity: ≥95% (HPLC)
Citations (12)
Reviews (1)
Literature (1)

Biological Activity for Nocodazole

Nocodazole is a microtubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.

Technical Data for Nocodazole

M. Wt 301.32
Formula C14H11N3O3S
Storage Store at RT
Purity ≥95% (HPLC)
CAS Number 31430-18-9
PubChem ID 4122
Smiles COC(=O)NC1=NC2=CC(=CC=C2N1)C(=O)C1=CC=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Nocodazole

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 15.07 50

Preparing Stock Solutions for Nocodazole

The following data is based on the product molecular weight 301.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 6.64 mL 33.19 mL 66.37 mL
2.5 mM 1.33 mL 6.64 mL 13.27 mL
5 mM 0.66 mL 3.32 mL 6.64 mL
25 mM 0.13 mL 0.66 mL 1.33 mL

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Product Datasheets for Nocodazole

Certificate of Analysis / Product Datasheet
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References for Nocodazole

References are publications that support the biological activity of the product.

Vasquez et al (1997) Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 8 973 PMID: 9201709

Webb et al (2004) Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 36 2541 PMID: 15325591

Mizushima et al (2010) Methods in mammalian autophagy research. Cell 140 313 PMID: 20144757

Lin et al (2014) Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 3 e04766 PMID: 25497837

If you know of a relevant reference for Nocodazole, please let us know.

Keywords: Nocodazole, Nocodazole supplier, Microtubule, inhibitors, inhibits, Tau, Tubulin, Microtubules, Mitosis, crispr, cas9, homology-directed, repair, HDR, Autophagy, CRISPR, Reagents, 1228, Tocris Bioscience

12 Citations for Nocodazole

Citations are publications that use Tocris products. Selected citations for Nocodazole include:

Paonessa et al (2019) Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia. Cell Rep 26 582 PMID: 30650353

Bernier et al (2019) Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep 27 2895 PMID: 31167136

Melemedjian et al (2014) Local translation and retrograde axonal transport of CREB regulates IL-6-induced nociceptive plasticity. Mol Pain 10 45 PMID: 24993495

Seese et al (2012) LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice. J Neurosci 32 7403 PMID: 22623686

Do you know of a great paper that uses Nocodazole from Tocris? Please let us know.

Reviews for Nocodazole

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Microtubule disruption.
By Anonymous on 01/30/2018
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: Brain slice

Used in brain slcies to disturb microtubule dynamics. Effect on cell shape was observed within minutes

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:

  • Cell Cycle and Mitosis
  • DNA Damage Repair
  • Targeted Protein Degradation
  • Ubiquitin Proteasome Pathway
  • Chemotherapy Targets