NIR Dye s775z, NHS

Pricing Availability   Qty
Description: Bright and photostable non-aggregating near-IR cyanine dye. Reactive group: NHS ester
Chemical Name: 2-((1E,3Z,5E)-4-(2,6-bis((4-(2,5,8,11-tetraoxadodecyl)-1H-1,2,3-triazol-1-yl)methyl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)phenyl)-7-((E)-3,3-dimethyl-5-sulfonato-1-(3-(trimethylammonio)propyl)indolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-dimethyl-1-(3-(trimethylammonio)propyl)-3H-indol-1-ium-5-sulfonate chloride
Purity: ≥90% (HPLC)
Protocols (1)
Literature (1)

Biological Activity for NIR Dye s775z, NHS

NIR Dye s775z, NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. When conjugated to antibodies, NIR Dye s775z, NHS is substantially brighter and more photostable than competitor dyes. It retains excellent target specificity, even when the degree of labeling (DOL) is very high. Excitation/emission maxima = 775/795 nm; extinction coefficient = 201000 M-1cm-1; quantum yield = 0.09.

For further information about how NIR Dye s775z, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Scientific Data

Fluorescence Imaging Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma. View Larger

Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma. HT-1080 cells were fixed, co-incubated with primary rabbit anti-tubulin overnight at 4 °C. The following day, they were incubated with secondary goat anti-rabbit labelled with NIR Dye s775z (green) for 2 h at room temperature. Cells were co-stained with Hoechst 33342 (blue, Catalog # 5117, Tocris). Dye used at 1:100 dilution. Scale: 30 μm. For more information on this work read Schreiber et al (2021) High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Anal.Chem. 93 3643 PMID: 33566567

In vivo Imaging Application of NIR Dye s775z, NHS in vivo View Larger

Application of NIR Dye s775z, NHS in vivo. Mock surgery process of a tumor bearing mouse 3h post retro-orbital co-injection of 100 μL (20 nmol) NIR Dye s775z, NHS conjugated to integrin receptor targeting cRGDfK peptide. Images were taken before the surgery, and after excising the tumor from mouse body. For more information on this work read Gamage et al (2021) Comparison of cRGDfK Peptide Probes with Appended Shielded Heptamethine Cyanine Dye (s775z) for Near Infrared Fluorescence Imaging of Cancer. ACS Omega 6 30130 PMID: 34778684

Licensing Information

Sold under license from the University of Notre Dame

Optical Data for NIR Dye s775z, NHS

NIR Dye s775z, NHS Dye Spectra
Emission Color Near-IR
λabs 775 nm
λem 795 nm
Extinction Coefficient (ε) 201000 M-1cm-1
Quantum Yield (φ) 0.09
Closest Laser line 750 nm
Reactive Group NHS ester
Reactivity Primary amines
Correction Factor 280 0.11
Application Fluorescence Microscopy, In Vivo Imaging
Alternative For FNIR-Tag, IRDye® 800CW, UL766

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for NIR Dye s775z, NHS

M. Wt 1507.22
Formula C72H100ClN11O18S2
Storage Store at -20°C
Purity ≥90% (HPLC)
Smiles CC1(C)C2=C(C=CC(S(=O)([O-])=O)=C2)N(CCC[N+](C)(C)C)/C1=C/C=C/C(C3=C(CN4C=C(COCCOCCOCCOC)N=N4)C=C(C(ON(C(CC5)=O)C5=O)=O)C=C3CN6N=NC(COCCOCCOCCOC)=C6)=C/C=C/C7=[N+](CCC[N+](C)(C)C)C8=C(C=C(S(=O)([O-])=O)C=C8)C7(C)C.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for NIR Dye s775z, NHS

References for NIR Dye s775z, NHS

References are publications that support the biological activity of the product.

Schreiber et al (2021) High-performance near-infrared fluorescent secondary antibodies for immunofluorescence. Anal.Chem. 93 3643 PMID: 33566567

If you know of a relevant reference for NIR Dye s775z, NHS, please let us know.

Keywords: NIR Dye s775z, NHS, NIR Dye s775z, NHS supplier, Dye, NHS, near, infrared, IR, Cyanine, NIR, fluorescent, dyes, imaging, amines, labeling, labelling, ester, Dyes, (Cy, Dyes), Near, Infrared, (NIR), Fluorescent, 7626, Tocris Bioscience

Citations for NIR Dye s775z, NHS

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Protocols for NIR Dye s775z, NHS

The following protocol features additional information for the use of NIR Dye s775z, NHS (Cat. No. 7626).

Literature in this Area

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*Please note that Tocris will only send literature to established scientific business / institute addresses.

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