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NIR Dye s775z, NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. When conjugated to antibodies, NIR Dye s775z, NHS is substantially brighter and more photostable than competitor dyes. It retains excellent target specificity, even when the degree of labeling (DOL) is very high. Excitation/emission maxima = 775/795 nm; extinction coefficient = 201000 M-1cm-1; quantum yield = 0.09.For further information about how NIR Dye s775z, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma. HT-1080 cells were fixed, co-incubated with primary rabbit anti-tubulin overnight at 4 °C. The following day, they were incubated with secondary goat anti-rabbit labelled with s775z (green) for 2 h at room temperature. Cells were co-stained with Hoechst 33342 (blue, Catalog # 5117, Tocris). Dye used at 1:100 dilution. Scale bar = 30 μm.
Application of NIR Dye s775z, NHS in vivo. Mock surgery process of a tumor bearing mouse 3h post retro-orbital co-injection of 100 μL (20 nmol) NIR Dye s775z, NHS conjugated to integrin receptor targeting cRGDfK peptide. Images were taken before the surgery, and after excising the tumor from mouse body.
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The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Schreiber et al (2021) High-performance near-infrared fluorescent secondary antibodies for immunofluorescence. Anal.Chem. 93 3643 PMID: 33566567
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Keywords: NIR Dye s775z, NHS, NIR Dye s775z, NHS supplier, Dye, NHS, near, infrared, IR, Cyanine, NIR, fluorescent, dyes, imaging, amines, labeling, labelling, ester, Dyes, (Cy, Dyes), Near, Infrared, (NIR), Fluorescent, 7626, Tocris Bioscience
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The following protocol features additional information for the use of NIR Dye s775z, NHS (Cat. No. 7626).
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: