Home / Pharmacology / Enzymes / Kinases / Receptor Interacting Protein Kinase Family / Receptor Interacting Protein Kinases / Receptor Interacting Protein Kinase Inhibitors / Necrostatin-1

Necrostatin-1

Cat. No. 2324 3 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Cat.No. 2324 - Necrostatin-1 | C13H13N3OS | CAS No. 4311-88-0
Description: RIP1 kinase inhibitor; inhibits necroptosis
Chemical Name: 5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone
Purity: ≥99% (HPLC)
Datasheet
Citations (3)
Reviews
Literature
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

ATP-competitive, allosteric inhibitor of receptor-interacting protein kinase 1 (RIPK1) (EC50 = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC50 = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.

Compound Libraries

Necrostatin-1 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 259.33
Formula C13H13N3OS
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 4311-88-0
PubChem ID 2828334
InChI Key TXUWMXQFNYDOEZ-UHFFFAOYSA-N
Smiles CN1C(=S)NC(CC2=CNC3=CC=CC=C23)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 115.7 30
ethanol 3.89 15 with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 259.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.3 mM 12.85 mL 64.27 mL 128.54 mL
1.5 mM 2.57 mL 12.85 mL 25.71 mL
3 mM 1.29 mL 6.43 mL 12.85 mL
15 mM 0.26 mL 1.29 mL 2.57 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the biological activity of the product.

Degterev et al (2005) Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat.Chem.Biol. 1 112 PMID: 16408008

Degterev et al (2008) Identification of RIP1 kinase as a specific cellular target of necroSTAT. Nat.Chem.Biol. 4 313 PMID: 18408713

Linkermann et al (2012) Rip1 (receptor-interacting protein kinase 1) mediates necroptosis and contributes to renal ischemia/reperfusion injury. 81 751 PMID: 22237751

If you know of a relevant reference for Necrostatin-1, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Receptor Interacting Protein Kinase Inhibitors

Keywords: Necrostatin-1, Necrostatin-1 supplier, non-apoptotic, cell, death, necroptosis, Death, domain, receptor-associated, adaptor, RIP1, inhibits, inhibitors, Apoptosis, RIPK, RIPK1, receptor-interacting, protein, kinase, 1, other, kinases, Necroptosis, RIP, Kinases, 2324, Tocris Bioscience

3 Citations for Necrostatin-1

Citations are publications that use Tocris products. Selected citations for Necrostatin-1 include:

Sagher et al (2014) The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells. Cancer Cell Int 14 75 PMID: 25324692

Kim (2017) Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells Journal of Translational Medicine 15 123 PMID: 28569199

Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618

Do you know of a great paper that uses Necrostatin-1 from Tocris? Please let us know.

Reviews for Necrostatin-1

There are currently no reviews for this product. Be the first to review Necrostatin-1 and earn rewards!

Have you used Necrostatin-1?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

New Product Guide

New Product Guide (Fall/Winter 2018/2019)

Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Chemogenetics
  • Epigenetics
  • Fluorescent Imaging
  • PROTACs
  • Signal Transduction
  • Stem Cells