Necrostatin-1

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Cat.No. 2324 - Necrostatin-1 | C13H13N3OS | CAS No. 4311-88-0
Description: RIP1 kinase inhibitor; inhibits necroptosis
Chemical Name: 5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone
Purity: ≥99% (HPLC)
Datasheet
Citations (3)
Reviews
Literature

Biological Activity

ATP-competitive, allosteric inhibitor of receptor-interacting protein kinase 1 (RIPK1) (EC50 = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC50 = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.

Compound Libraries

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Technical Data

M. Wt 259.33
Formula C13H13N3OS
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 4311-88-0
PubChem ID 2828334
InChI Key TXUWMXQFNYDOEZ-UHFFFAOYSA-N
Smiles CN1C(=S)NC(CC2=CNC3=CC=CC=C23)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 115.7 30
ethanol 3.89 15 with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 259.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.3 mM 12.85 mL 64.27 mL 128.54 mL
1.5 mM 2.57 mL 12.85 mL 25.71 mL
3 mM 1.29 mL 6.43 mL 12.85 mL
15 mM 0.26 mL 1.29 mL 2.57 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Degterev et al (2005) Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat.Chem.Biol. 1 112 PMID: 16408008

Degterev et al (2008) Identification of RIP1 kinase as a specific cellular target of necroSTAT. Nat.Chem.Biol. 4 313 PMID: 18408713

Linkermann et al (2012) Rip1 (receptor-interacting protein kinase 1) mediates necroptosis and contributes to renal ischemia/reperfusion injury. 81 751 PMID: 22237751


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View Related Products by Product Action

View all Receptor Interacting Protein Kinase Inhibitors

Keywords: Necrostatin-1, Necrostatin-1 supplier, non-apoptotic, cell, death, necroptosis, Death, domain, receptor-associated, adaptor, RIP1, inhibits, inhibitors, Apoptosis, RIPK, RIPK1, receptor-interacting, protein, kinase, 1, other, kinases, Necroptosis, RIP, Kinases, 2324, Tocris Bioscience

3 Citations for Necrostatin-1

Citations are publications that use Tocris products. Selected citations for Necrostatin-1 include:

Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618

Kim (2017) Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells Journal of Translational Medicine 15 123 PMID: 28569199

Sagher et al (2014) The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells. Cancer Cell Int 14 75 PMID: 25324692


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Literature in this Area

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