MRT 10

Pricing Availability Delivery Time Qty
Cat.No. 4585 - MRT 10 | C24H23N3O5S | CAS No. 330829-30-6
Description: Smoothened (Smo) antagonist
Chemical Name: N-[[[3-Benzoylamino)phenyl]amino]thioxomethyl]-3,4,5-trimethoxybenzamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Smoothened (Smo) receptor antagonist (IC50 = 0.5 μM in HEK293 cells transiently expressing mouse Smo). Inhibits ShhN-induced Gli luciferase reporter activity in Shh-light 2 cells (IC50 = 2.5 μM); acts as an inverse agonist during nontranscriptional Hedgehog pathway activation (IC50 = 2.5 μM in HEK293 cells).

Technical Data

M. Wt 465.52
Formula C24H23N3O5S
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 330829-30-6
PubChem ID 1139102
InChI Key KVQVEZQDNHMQJV-UHFFFAOYSA-N
Smiles O=C(NC(NC2=CC(NC(C3=CC=CC=C3)=O)=CC=C2)=S)C1=CC(OC)=C(OC)C(OC)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 46.55 100

Preparing Stock Solutions

The following data is based on the product molecular weight 465.52. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.15 mL 10.74 mL 21.48 mL
5 mM 0.43 mL 2.15 mL 4.3 mL
10 mM 0.21 mL 1.07 mL 2.15 mL
50 mM 0.04 mL 0.21 mL 0.43 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Solinas et al (2012) Acylthiourea, acylurea, and acylguanidine derivatives with potent hedgehog inhibiting activity. J.Med.Chem. 55 1559 PMID: 22268551

Manetti et al (2010) Virtual screening-based discovery and mechanistic characterization of the acylthiourea MRT-10 family as smoothened antagonists. Mol.Pharmacol. 78 658 PMID: 20664000


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Keywords: MRT10 smoothened receptor antagonists inhibitors inhibits hedgehog pathway inverse agonists nontranscriptional Gli Shh sonic Smoothened Receptors

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GPCR

GPCR Product Listing

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Pathways for MRT 10

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