ML 335

Pricing Availability   Qty
Description: Selective K2P2.1 (TREK-1) and K2P10.1 (TREK-2) activator
Chemical Name: N-[(2,4-Dichlorophenyl)methyl]-4-[(methylsulfonyl)amino]benzamide
Purity: ≥98% (HPLC)
Citations (2)

Biological Activity for ML 335

ML 335 is a selective K2P2.1 (TREK-1; KCNK2) and K2P10.1 (TREK-2; KCNK10) activator (EC50 values of 14.3 μM and 5.2 μM, respectively); directly stimulates the C-type gate via the K2P modulator pocket. Does not activate K2P4.1 (TRAAK).

Technical Data for ML 335

M. Wt 373.25
Formula C15H14Cl2N2O3S
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 825658-06-8
PubChem ID 1243054
Smiles CS(=O)(NC1=CC=C(C(NCC2=C(C=C(C=C2)Cl)Cl)=O)C=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ML 335

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 37.33 100
ethanol 7.46 20

Preparing Stock Solutions for ML 335

The following data is based on the product molecular weight 373.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.68 mL 13.4 mL 26.79 mL
5 mM 0.54 mL 2.68 mL 5.36 mL
10 mM 0.27 mL 1.34 mL 2.68 mL
50 mM 0.05 mL 0.27 mL 0.54 mL

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References for ML 335

References are publications that support the biological activity of the product.

Pope et al (2018) Protein and chemical determinants of BL-1249 action and selectivity for K2P channels. ACS Chem.Neurosci. 9 3153 PMID: 30089357

Gada et al (2019) Two-pore domain potassium channels: emerging targets for novel analgesic drugs: IUPHAR Review 26. Br.J.Pharmacol. 176 256 PMID: 30325008

Lolicato et al (2017) K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature 547 364 PMID: 28693035

Schewe et al (2019) A pharmacological master key mechanism that unlocks the selectivity filter gate in K+ channels. Science 363 875 PMID: 30792303

If you know of a relevant reference for ML 335, please let us know.

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Keywords: ML 335, ML 335 supplier, ML335, Selective, K2P2.1, TREK-1, KCNK2, K2P10.1, TREK-2, KCNK10, activators, activates, Two-P, Potassium, Channels, 6887, Tocris Bioscience

2 Citations for ML 335

Citations are publications that use Tocris products. Selected citations for ML 335 include:

Lei et al (2020) Recombinant CCL17 Enhances Hematoma Resolution and Activation of CCR4/ERK/Nrf2/CD163 Signaling Pathway After Intracerebral Hemorrhage in Mice. Neurotherapeutics 17 1940-1953 PMID: 32783091

Peng et al (2021) Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats. Oxid Med Cell Longev 2021 8891373 PMID: 33708336

Do you know of a great paper that uses ML 335 from Tocris? Please let us know.

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