Mitoxantrone dihydrochloride

Pricing Availability Delivery Time Qty
Cat.No. 4250 - Mitoxantrone dihydrochloride | C22H28N4O6.2HCl | CAS No. 70476-82-3
Description: Topoisomerase II inhibitor; immunosuppressive and antineoplastic agent
Chemical Name: 1,4-Dihydroxy-5,8-bis[[2-[(2-hydroxyethyl)amino]ethyl]amino]-9,10-anthracenedione dihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Literature

Biological Activity

Type II DNA topoisomerase inhibitor. Disrupts DNA synthesis and repair and induces damage by DNA cross-linking. Also inhibits PIM1 kinase (IC50 = 51 nM). Immunomodulatory, antineoplastic and chemotherapeutic agent. Also USP11 inhibitor (IC50= 3.15 μM). Induces cell death of pancreatic cancer cell lines expressing wild-type BRCA2.

Compound Libraries

Mitoxantrone dihydrochloride is also offered as part of the Tocriscreen Epigenetics Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 517.4
Formula C22H28N4O6.2HCl
Storage Desiccate at RT
Purity ≥98% (HPLC)
CAS Number 70476-82-3
PubChem ID 51082
InChI Key ZAHQPTJLOCWVPG-UHFFFAOYSA-N
Smiles O=C2C3=C(C(O)=CC=C3O)C(C1=C(NCCNCCO)C=CC(NCCNCCO)=C12)=O.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.8 75
water 2.59 5

Preparing Stock Solutions

The following data is based on the product molecular weight 517.4. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.93 mL 9.66 mL 19.33 mL
5 mM 0.39 mL 1.93 mL 3.87 mL
10 mM 0.19 mL 0.97 mL 1.93 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Scott and Figgit (2004) Mitoxantrone: a review of its uses in multiple sclerosis. CNS Drugs. 18 379 PMID: 15089110

Crespi et al (1986) Mitoxantrone affects topoisomerase activities in human breast cancer cells. Biochem.Biophys.Res.Comm. 136 521 PMID: 3010982

Wan et al (2013) A new target for an old drug: identifying mitoxantrone as a nanomolar inhibitor of PIM1 kinase via kinome-wide selectivity modeling. J.Med.Chem. 56 2619 PMID: 23442188

Burkhart et al (2013) Mitoxantrone targets human ubiquitin-specific peptidase 11 (USP11) and is a potent inhibitor of pancreatic cancer cell survival. Mol.Cancer Res. 11 901 PMID: 23696131


If you know of a relevant reference for Mitoxantrone dihydrochloride, please let us know.

View Related Products by Product Action

View all DNA Topoisomerase Inhibitors

Keywords: topoisomerase II inhibitors inhibits DNA topoisomerase type II antitumor immunosuppressant DNA synthesis repair PIM1 kinase USP11 ubiquitin specific peptidase 11 chemotherapeutics DNA Topoisomerase

1 Citation for Mitoxantrone dihydrochloride

Citations are publications that use Tocris products. Selected citations for Mitoxantrone dihydrochloride include:

Zhang (2017) The BTK Inhibitor, Ibrutinib (PCI-32765) Overcomes Paclitaxel Resistance in ABCB1 and ABCC10 Overexpressing Cells and Tumors. Mol Cancer Ther 16 1021 PMID: 28265007


Do you know of a great paper that uses Mitoxantrone dihydrochloride from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Mitoxantrone dihydrochloride

Protocols

TODO: Add Protocols