Inhibitor of PARP-3 (IC50 = 0.89 μM). Displays selectivity for PARP-3 over PARP-1, PARP-2 and other ARDT enzymes (IC50 values are 6.3, 10.8 and >30 μM respectively). Enhances CRISPR-Cas9-mediated HER2 mutation frequency, resulting in increased reduction in proliferation of HER2-positive breast cancer cells. Cell permeable.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 321.37. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.11 mL||15.56 mL||31.12 mL|
|5 mM||0.62 mL||3.11 mL||6.22 mL|
|10 mM||0.31 mL||1.56 mL||3.11 mL|
|50 mM||0.06 mL||0.31 mL||0.62 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Lindgren et al (2013) PARP inhibitor with selectivity toward ADP-ribosyltransferase ARTD3/PARP3. ACS Chem.Biol. 8 1698 PMID: 23742272
Wang and Sun (2016) CRISPR-mediated targeting of HER2 inhibits cell proliferation through a dominant negative mutation. Cancer Lett. PMID: 27815036
If you know of a relevant reference for ME 0328, please let us know.
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Keywords: ME0328 PARP3 ARDT3 inhibitors inhibits selective CRISPR-Cas9 HER2 Poly(ADP-ribose) Polymerase
1 Citation for ME 0328
Citations are publications that use Tocris products. Selected citations for ME 0328 include:
Wang and Sun et al (2016) CRISPR-mediated targeting of HER2 inhibits cell proliferation through a dominant negative mutation. Cancer Letters 385 137 PMID: 27815036
Do you know of a great paper that uses ME 0328 from Tocris? If so please let us know.
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In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.