KH 7

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Cat.No. 3834 - KH 7 | C17H15BrN4O2S | CAS No. 330676-02-3
Description: Selective soluble adenylyl cyclase inhibitor
Chemical Name: (±)-2-(1H-benzimidazol-2-ylthio)propanoic acid 2-[(5-bromo-2-hydroxyphenyl)methylene]hydrazide
Purity: ≥99% (HPLC)
Datasheet
Citations (9)
Reviews (1)
Literature (1)

Biological Activity

Selective soluble adenylyl cyclase (sAC) inhibitor (IC50 = 3 - 10 μM in vivo). Inert towards transmembrane adenylyl cyclase (tmAC) in vitro and in whole cells at concentrations up to 300 μM. Blocks synthesis of cAMP and displays an antiapoptotic effect at concentrations of 1 - 100 μM.

Technical Data

M. Wt 419.3
Formula C17H15BrN4O2S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 330676-02-3
PubChem ID 6843191
InChI Key GEHVZUUHMWNMAY-UHFFFAOYSA-N
Smiles OC(C=CC(Br)=C2)=C2/C=N/NC(C(C)SC1=NC(C=CC=C3)=C3N1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 41.93 100

Preparing Stock Solutions

The following data is based on the product molecular weight 419.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.38 mL 11.92 mL 23.85 mL
5 mM 0.48 mL 2.38 mL 4.77 mL
10 mM 0.24 mL 1.19 mL 2.38 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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References

References are publications that support the biological activity of the product.

Kumar et al (2009) Soluble adenylyl cyclase controls mitochondria-dependent apoptosis in coronary endothelial cells. J.Biol.Chem. 284 14760 PMID: 19336406

Stessin et al (2006) Soluble adenylyl cyclase mediates nerve growth factor-induced activation of Rap1. J.Biol.Chem. 281 17253 PMID: 16627466

Hess et al (2005) The 'soluble' adenylyl cyclase in sperm mediates multiple signaling events required for fertilization. Develop.Cell 9 249


If you know of a relevant reference for KH 7, please let us know.

View Related Products by Product Action

View all Adenylyl Cyclase Inhibitors

Keywords: KH 7, KH 7 supplier, selective, adenylyl, cyclases, inhibitors, inhibits, soluble, sAC, KH7, Adenylyl, Cyclase, 3834, Tocris Bioscience

9 Citations for KH 7

Citations are publications that use Tocris products. Selected citations for KH 7 include:

Shahidullah et al (2014) Nonpigmented ciliary epithelial cells respond to acetazol. by a soluble adenylyl cyclase mechanism. Invest Ophthalmol Vis Sci 55 187 PMID: 24282227

Inda et al (2017) cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells. Sci Rep 7 1944 PMID: 28512295

Inda et al (2016) Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling. J Cell Biol 214 181 PMID: 27402953

Chen et al (2013) Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway. Mediators Inflamm 9 2033 PMID: 24145604

Jahchan et al (2013) A drug repositioning approach identifies tricyclic antidepressants as inhibitors of small cell lung cancer and other neuroendocrine tumors. Cancer Discov 3 1364 PMID: 24078773

Lee et al (2012) The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature 492 123 PMID: 23143333

Yamada et al (2018) Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation. J Reprod Dev 64 377 PMID: 29952339

Schnúr et al (2019) Phosphorylation-dependent modulation of CFTR macromolecular signalling complex activity by cigarette smoke condensate in airway epithelia. Sci Rep 9 12706 PMID: 31481727

Ishii et al (2019) An improved method for isolation of mitochondria from cell lines that enables reconstitution of calcium-dependent processes. Anal Biochem 577 52 PMID: 31022379


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Reviews for KH 7

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a selective sAC inhibitor.
By Hyun Choi on 01/31/2018
Assay Type: Ex Vivo
Species: Rat

Used KH7 to block soluble adenylyl cyclase which is sensitive to bicarbonate. Used at 20uM in rat brain slice.

pretreat KH7 at least 30 min before treatment


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