JIB 04

Pricing Availability   Qty
Description: Pan Jumonji inhibitor; active in vivo
Chemical Name: 5-Chloro-2-[(E)-2-[phenyl(pyridin-2-yl)methylidene]hydrazin-1-yl]pyridine
Purity: ≥99% (HPLC)
Citations (7)
Literature (1)

Biological Activity for JIB 04

JIB 04 is a pan Jumonji histone demethylase inhibitor (IC50 values are 230, 340, 435, 445, 855 and 1100 nM for JARID1A, JMJD2E, JMJD2B, JMJD2A, JMJD3 and JMJD2C respectively). Exhibits negligible activity at PHD2 and TET1. Selectively blocks cancer cell growth in vitro and diminishes tumor growth in H358 and A549 mouse xenograft models in vivo. Prolongs survival in a mouse model of breast cancer.

Compound Libraries for JIB 04

JIB 04 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for JIB 04

M. Wt 308.76
Formula C17H13ClN4
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 199596-05-9
PubChem ID 6519698
Smiles ClC(C=C3)=CN=C3N/N=C(C2=CC=CC=C2)/C1=CC=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for JIB 04

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 30.88 100

Preparing Stock Solutions for JIB 04

The following data is based on the product molecular weight 308.76. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.24 mL 16.19 mL 32.39 mL
5 mM 0.65 mL 3.24 mL 6.48 mL
10 mM 0.32 mL 1.62 mL 3.24 mL
50 mM 0.06 mL 0.32 mL 0.65 mL

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Product Datasheets for JIB 04

Certificate of Analysis / Product Datasheet
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References for JIB 04

References are publications that support the biological activity of the product.

Wang et al (2013) A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth. Nat. Commun. 4 2035 PMID: 23792809

Grunicke and Hofmann (1997) Azinyl and diazinyl hydrazones derived from aryl N-heteroaryl ketones: synthesis and antiproliferative activity. J. Med. Chem. 40 4420 PMID: 9435912

If you know of a relevant reference for JIB 04, please let us know.

View Related Products by Target

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View all Histone Demethylase Inhibitors

Keywords: JIB 04, JIB 04 supplier, JIB04, histone, demethylases, inhibits, inhibitors, JMJ, JARID1A, JMJD2A, JMJD2B, JMJD2C, JMJD2E, JMJD3, pan, selective, antiproliferative, Histone, Demethylases, 4972, Tocris Bioscience

7 Citations for JIB 04

Citations are publications that use Tocris products. Selected citations for JIB 04 include:

Poulard et al (2018) Increasing G9a automethylation sensitizes B acute lymphoblastic leukemia cells to glucocorticoid-induced death. Cell Death Dis 9 1038 PMID: 30305606

Rowbotham et al (2018) H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression. Nat Commun 9 4559 PMID: 30455465

Jin Kyung et al (2021) BIX01294 inhibits EGFR signaling in EGFR-mutant lung adenocarcinoma cells through a BCKDHA-mediated reduction in the EGFR level. Exp Mol Med 53 1877-1887 PMID: 34876693

Jan E et al (2021) Inhibitor of growth protein 3 epigenetically silences endogenous retroviral elements and prevents innate immune activation. Nucleic Acids Res 49 12706-12715 PMID: 34791430

Do you know of a great paper that uses JIB 04 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Epigenetics Scientific Review

Epigenetics Scientific Review

Written by Susanne Müller-Knapp and Peter J. Brown, this review gives an overview of the development of chemical probes for epigenetic targets, as well as the impact of these tool compounds being made available to the scientific community. In addition, their biological effects are also discussed. Epigenetic compounds available from Tocris are listed.