JIB 04

Pricing Availability Delivery Time Qty
Cat.No. 4972 - JIB 04 | C17H13ClN4 | CAS No. 199596-05-9
Description: Pan Jumonji inhibitor; active in vivo
Chemical Name: 5-Chloro-2-[(E)-2-[phenyl(pyridin-2-yl)methylidene]hydrazin-1-yl]pyridine
Purity: ≥99% (HPLC)
Datasheet
Citations (1)
Literature

Biological Activity

Pan Jumonji histone demethylase inhibitor (IC50 values are 230, 340, 435, 445, 855 and 1100 nM for JARID1A, JMJD2E, JMJD2B, JMJD2A, JMJD3 and JMJD2C respectively). Exhibits negligible activity at PHD2 and TET1. Selectively blocks cancer cell growth in vitro and diminishes tumor growth in H358 and A549 mouse xenograft models in vivo. Prolongs survival in a mouse model of breast cancer.

Compound Libraries

JIB 04 is also offered as part of the Tocriscreen Plus and Tocriscreen Epigenetics Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 308.76
Formula C17H13ClN4
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 199596-05-9
PubChem ID 6519698
InChI Key YHHFKWKMXWRVTJ-OQKWZONESA-N
Smiles ClC(C=C3)=CN=C3N/N=C(C2=CC=CC=C2)/C1=CC=CC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
1eq. HCl 15.44 50
DMSO 30.88 100

Preparing Stock Solutions

The following data is based on the product molecular weight 308.76. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.24 mL 16.19 mL 32.39 mL
5 mM 0.65 mL 3.24 mL 6.48 mL
10 mM 0.32 mL 1.62 mL 3.24 mL
50 mM 0.06 mL 0.32 mL 0.65 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Wang et al (2013) A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth. Nat. Commun. 4 2035 PMID: 23792809

Grunicke and Hofmann (1997) Azinyl and diazinyl hydrazones derived from aryl N-heteroaryl ketones: synthesis and antiproliferative activity. J. Med. Chem. 40 4420 PMID: 9435912


If you know of a relevant reference for JIB 04, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Histone Demethylase Inhibitors

Keywords: JIB 04, supplier, JIB04, histone, demethylases, inhibits, inhibitors, JMJ, JARID1A, JMJD2A, JMJD2B, JMJD2C, JMJD2E, JMJD3, pan, selective, antiproliferative, Histone, Demethylases, Tocris Bioscience

1 Citation for JIB 04

Citations are publications that use Tocris products. Selected citations for JIB 04 include:

Lee et al (2016) Signalling couples hair follicle stem cell quiescence with reduced histone H3 K4/K9/K27me3 for proper tissue homeostasis. Nat Commun 7 11278 PMID: 27080563


Do you know of a great paper that uses JIB 04 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Epigenetics

Epigenetics Scientific Review

Written by Susanne Müller-Knapp and Peter J. Brown, this review gives an overview of the development of chemical probes for epigenetic targets, as well as the impact of these tool compounds being made available to the scientific community. In addition, their biological effects are also discussed. Epigenetic compounds available from Tocris are listed.

Epigenetics

Epigenetics Research Bulletin

Produced by Tocris and updated in 2014, the epigenetics research bulletin gives an introduction into mechanisms of epigenetic regulation, and highlights key Tocris products for epigenetics targets including:

  • Bromodomains
  • DNA Methyltransferases
  • Histone Deacetylases
  • Histone Demethylases
  • Histone Methyltransferases

Pathways for JIB 04

Protocols

TODO: Add Protocols