JB 300

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Description: Aurora A Degrader (PROTAC®)
Chemical Name: trans-4-(3-Chloro-2-fluorophenoxy)-N-(2-(2-(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethoxy)ethoxy)ethyl)-1-((6-(thiazol-2-ylamino)pyridin-2-yl)methyl)cyclohexane-1-carboxamide
Purity: ≥98% (HPLC)
Literature (4)

Biological Activity for JB 300

JB 300 is a potent and selective Aurora A Degrader (PROTAC®; DC50 = 30 nM and Dmax = 78%). It comprises a cereblon E3-ligase ligand Thalidomide (Cat. No. 0652) joined by a PEG2 linker to the Aurora A inhibitor MK-5108. The compound depletes Aurora A in MV4-11 cell lines and exhibits no degradation of Aurora B at 10 µM. JB 300 shows dose-dependent cytotoxicity in AML cells.

Aurora A antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF3295 and NBP1-51843.

PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold under license from University of Würzburg

Technical Data for JB 300

M. Wt 906.38
Formula C43H45ClFN7O10S
Storage Store at -20°C
Purity ≥98% (HPLC)
Smiles O=C([C@@]1(CC2=CC=CC(NC3=NC=CS3)=N2)CC[C@H](OC4=C(F)C(Cl)=CC=C4)CC1)NCCOCCOCCNC(COC5=C6C(N(C7CCC(NC7=O)=O)C(C6=CC=C5)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for JB 300

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 18.13 20

Preparing Stock Solutions for JB 300

The following data is based on the product molecular weight 906.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 5.52 mL 27.58 mL 55.16 mL
1 mM 1.1 mL 5.52 mL 11.03 mL
2 mM 0.55 mL 2.76 mL 5.52 mL
10 mM 0.11 mL 0.55 mL 1.1 mL

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References for JB 300

References are publications that support the biological activity of the product.

Adhikari et al (2020) PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase. Nat.Chem.Biol. 16 1179 PMID: 32989298

Bozolovic et al (2022) Novel, highly potent PROTACs targeting AURORA-A kinase. Curr.Res.Chem.Biol. 2 100032

If you know of a relevant reference for JB 300, please let us know.

Keywords: JB 300, JB 300 supplier, JB300, AURORA-A, PROTACs, Degraders, degrades, kinase., Aurora, Kinases, Protein, 7837, Tocris Bioscience

Citations for JB 300

Citations are publications that use Tocris products.

Currently there are no citations for JB 300. Do you know of a great paper that uses JB 300 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Targeted Protein Degradation Research Product Guide

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Ubiquitin-Proteasome System Proteins
  • Assays for Protein Degradation
Epigenetics Scientific Review

Epigenetics Scientific Review

Written by Susanne Müller-Knapp and Peter J. Brown, this review gives an overview of the development of chemical probes for epigenetic targets, as well as the impact of these tool compounds being made available to the scientific community. In addition, their biological effects are also discussed. Epigenetic compounds available from Tocris are listed.

Epigenetics in Cancer Poster

Epigenetics in Cancer Poster

This poster summarizes the main epigenetic targets in cancer. The dysregulation of epigenetic modifications has been shown to result in oncogenesis and cancer progression. Unlike genetic mutations, epigenetic alterations are considered to be reversible and thus make promising therapeutic targets.

Targeted Protein Degradation Poster

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia