Floxuridine

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Cat.No. 4659 - Floxuridine | C9H11FN2O5 | CAS No. 50-91-9
Description: Thymidylate synthetase inhibitor
Alternative Names: FdUrd
Chemical Name: 5-Fluoro-2'-deoxyuridine
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews
Literature (2)
Pathways (1)

Biological Activity

Antineoplastic antimetabolite. Exhibits antiproliferative activity; inhibits thymidylate synthetase and disrupts DNA replication in human cells. Induces double-strand DNA breaks; activates ATR and ATM signaling pathways. Induces phosphorylation of Chk1 and Chk2 in OVCAR-8 and SKOV3ip ovarian cancer cell lines.

Technical Data

M. Wt 246.19
Formula C9H11FN2O5
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 50-91-9
PubChem ID 5790
InChI Key ODKNJVUHOIMIIZ-RRKCRQDMSA-N
Smiles O[C@H]1C[C@H](N2C=C(F)C(NC2=O)=O)O[C@@H]1CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 24.62 100
DMSO 24.62 100

Preparing Stock Solutions

The following data is based on the product molecular weight 246.19. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.06 mL 20.31 mL 40.62 mL
5 mM 0.81 mL 4.06 mL 8.12 mL
10 mM 0.41 mL 2.03 mL 4.06 mL
50 mM 0.08 mL 0.41 mL 0.81 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Huehls et al (2012) Poly(ADP-ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells. Cancer Res. 71 4944 PMID: 21613406

Bapat et al (1983) Human leukemic cells resistant to 5-fluoro-2'-deoxyuridine contain a thymidylate synthetase with lower affinity for nucleotides. J.Biol.Chem. 258 4130 PMID: 6220000

Wyatt and Wilson (2009) Participation of DNA repair in the response to 5-fluorouracil. Cell.Mol.Life Sci. 66 788 PMID: 18979208


If you know of a relevant reference for Floxuridine, please let us know.

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Keywords: Floxuridine, Floxuridine supplier, FdUrd, antiproliferatives, antineoplastic, antimetabolites, thymidylate, synthetases, synthases, inhibititors, DNA, synthesis, chemotherapeutics, DNA,, RNA, and, Protein, Synthesis, Thymidylate, Synthetase, 4659, Tocris Bioscience

1 Citation for Floxuridine

Citations are publications that use Tocris products. Selected citations for Floxuridine include:

Tiwari et al (2017) Zika virus infection reprograms global transcription of host cells to allow sustained infection. Emerg Microbes Infect 6 e24 PMID: 28442752


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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer Metabolism

Cancer Metabolism Poster

Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the main targets for cancer metabolism researchers. Genetic changes and epigenetic modifications in cancer cells alter the regulation of cellular metabolic pathways. These distinct metabolic circuits could provide viable cancer therapeutic targets.

Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Floxuridine

Apoptosis

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.
p53

p53 Signaling Pathway

p53 signaling plays an important role in the co-ordination of the cellular response different types of stress such as DNA damage and hypoxia. The downstream signals lead to apoptosis, senescence and cell cycle arrest.