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Description: H+-ATPase activator; activates autophagy; inactivates mTORC1 signaling
Chemical Name: N-[4-Fluoro-3-[(1-oxo-2-propen-1-yl)amino]phenyl]-1-(2-fluorophenyl)-1H-pyrazole-4-carboxamide
Purity: ≥98% (HPLC)

Biological Activity for EN6

EN6 is an activator of lysosomal vacuolar H+ ATPase (v-ATPase). EN6 covalently binds to the catalytic subunit of v-ATPase leading to increased acidification of lysosomes, inactivation of mTORC1 signaling and activation of autophagy in vivo. EN6 triggers entosis and promotes clearance of TDP43 protein aggregates in vitro.

Technical Data for EN6

M. Wt 368.34
Formula C19H14F2N4O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1808714-73-9
PubChem ID 99640033
Smiles O=C(C=C)NC1=CC(=CC=C1F)NC(=O)C=2C=NN(C2)C=3C=CC=CC3F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for EN6

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.68 10

Preparing Stock Solutions for EN6

The following data is based on the product molecular weight 368.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 27.15 mL 135.74 mL 271.49 mL
0.5 mM 5.43 mL 27.15 mL 54.3 mL
1 mM 2.71 mL 13.57 mL 27.15 mL
5 mM 0.54 mL 2.71 mL 5.43 mL

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References for EN6

References are publications that support the biological activity of the product.

Chung et al (2019) Covalent targeting of the vacuolar H+-ATPase activates autophagy via mTORC1 inhibition. Nat.Chem.Biol. 15 776 PMID: 31285595

Su et al (2021) Role and dynamics of vacuolar pH during cell-in-cell mediated death. Cell Death Dis. 12 119 PMID: 33483474

If you know of a relevant reference for EN6, please let us know.

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Keywords: EN6, EN6 supplier, EN6, activators, activates, autophagy, mTORC1signaling, lysosomes, lysosomal, vacuolar, H+, ATPases, entosis, v-ATPase, mTORC1, Autophagy, H+-ATPase, 7474, Tocris Bioscience

Citations for EN6

Citations are publications that use Tocris products.

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