EHNA hydrochloride

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Cat.No. 1261 - EHNA hydrochloride | C14H23N5O.HCl | CAS No. 58337-38-5
Description: Adenosine deaminase inhibitor
Chemical Name: erythro-9-(2-Hydroxy-3-nonyl)adenine hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (6)
Literature

Biological Activity

Selective inhibitor of cGMP-stimulated phosphodiesterase (PDE2) (IC50 = 0.8 - 4 μM). Also a potent inhibitor of adenosine deaminase. Suppresses spontaneous differentiation of human ESCs in feeder-free conditions. Also prevents directed neuronal differentiation.

Technical Data

M. Wt 313.83
Formula C14H23N5O.HCl
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 58337-38-5
PubChem ID 11056106
InChI Key VVDXNJRUNJMYOZ-DHXVBOOMSA-N
Smiles NC1=NC=NC2=C1N=CN2[C@@H](CCCCCC)[C@H](O)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 31.38 100
ethanol 31.38 100
water 31.38 100

Preparing Stock Solutions

The following data is based on the product molecular weight 313.83. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.19 mL 15.93 mL 31.86 mL
5 mM 0.64 mL 3.19 mL 6.37 mL
10 mM 0.32 mL 1.59 mL 3.19 mL
50 mM 0.06 mL 0.32 mL 0.64 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Bessodes et al (1982) Effect of chirality in erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) on adenosine deaminase inhibition. Biochem.Pharmacol. 31 879 PMID: 7082355

Michie et al (1996) Rapid regulation of PDE-2 and PDE-4 cyclic AMP phosphodiesterase activity following ligation of the T-cell antigen receptor on thymocytes: analysis using the selective inhibitors erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA) and rolipram. Cell.Signal. 8 97 PMID: 8730511

Podzuweit et al (1995) Isozyme selective inhibition of cGMP-stimulated cyclic nucleotide phosphodiesterases by erythro-9-(2-hydroxy-3-nonyl)adenine. Cell.Signal. 7 733 PMID: 8519602


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Keywords: EHNA hydrochloride, supplier, PDE2, inhibitors, inhibits, adenosines, deaminases, Phosphodiesterases, Deaminases, Tocris Bioscience

6 Citations for EHNA hydrochloride

Citations are publications that use Tocris products. Selected citations for EHNA hydrochloride include:

Prakasam et al (2012) Modulation of bladder function by luminal adenosine turnover and A1 receptor activation. PLoS One 303 F279 PMID: 22552934

Takahashi et al (2010) Adenosine and inosine release during hypoxia in the isolated spinal cord of neonatal rats. Br J Pharmacol 161 1806 PMID: 20735412

Davidson et al (2016) Alkaline Phosphatase, Soluble Extracellular Adenine Nucleotides, and Adenosine Production after Infant Cardiopulmonary Bypass PLoS One 11 e0158981 PMID: 27384524

Li et al (2015) Inhibition of adenosine deaminase (ADA)-mediated metabolism of cordycepin by natural substances. Am J Physiol Renal Physiol 3 e00121 PMID: 26038697

Liu et al (2014) Detection of oxidation products of 5-methyl-2'-deoxycytidine in Arabidopsis DNA. Nat Neurosci 8 e84620 PMID: 24391970

Guerrero et al (2013) Induction of 8,5'-cyclo-2'-deoxyadenosine and 8,5'-cyclo-2'-deoxyguanosine in isolated DNA by Fenton-type reagents. Chem Res Toxicol 26 1361 PMID: 23961697


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