EDA-DA

Pricing Availability   Qty
Cat.No. 7714 - EDA-DA | C8H12N2O3.CF3CO2H | CAS No. 87156-01-2
Description: Unnatural dipeptide building block with alkyne group for functionalizing peptidoglycan
Chemical Name: N-(4,4,5,5-Tetradehydro-D-norvalyl)-D-alanine trifluoroacetate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for EDA-DA

EDA-DA is an unnatural dipeptide building block (an ethynyl-D-alanine and a D-alanine). It incorporates a biorthogonal alkyne group into peptidoglycan (PG) through MurF in the cytoplasmic pathway, which enables selective labeling via a click-chemistry reaction. EDA-DA allows labeling of PG in Gram-positive (B. subtilis), Gram-negative (E. coli and C. trachomatis), Mycobacterium (M. smegmatis) and moss plastids (P. patens) with azide modified fluorescent dyes such as Alexa Fluor 488.

Technical Data for EDA-DA

M. Wt 298.22
Formula C8H12N2O3.CF3CO2H
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 87156-01-2
InChI Key BRIXKPONTJDKNO-KGZKBUQUSA-N
Smiles C[C@H](C(O)=O)NC([C@@H](CC#C)N)=O.FC(F)(F)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for EDA-DA

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 29.82 100
water 29.82 100

Preparing Stock Solutions for EDA-DA

The following data is based on the product molecular weight 298.22. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.35 mL 16.77 mL 33.53 mL
5 mM 0.67 mL 3.35 mL 6.71 mL
10 mM 0.34 mL 1.68 mL 3.35 mL
50 mM 0.07 mL 0.34 mL 0.67 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for EDA-DA

References are publications that support the biological activity of the product.

Cheung et al (1983) Chloralanyl and propargylglycyl dipeptides. Suicide substrate containing antibacterials. J.Med.Chem. 26 1733 PMID: 6417332

Liechti et al (2013) A new metabolic cell-wall labelling method reveals peptidoglycan in Chlamydia trachomatis. Nature 506 507 PMID: 24336210

Hirano et al (2016) Moss chloroplasts are surrounded by a peptidoglycan wall containing D-amino acids. Plant Cell 28 1521 PMID: 27325639

Kuru et al (2019) Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis. ACS Chem.Biol. 14 2745 PMID: 31743648

Banahene et al (2022) Chemical reporters for bacterial glycans: development and applications. Chem.Rev. 122 3336 PMID: 34905344


If you know of a relevant reference for EDA-DA, please let us know.

Keywords: EDA-DA, EDA-DA supplier, unnatural, dipeptide, ethynyl-D-alanine, alkyne, selective, labeling, peptidoglycan, Gram-positive, Gram-negative, Mycobacterium, bacteria, Fluorescent, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 7714, Tocris Bioscience

Citations for EDA-DA

Citations are publications that use Tocris products.

Currently there are no citations for EDA-DA. Do you know of a great paper that uses EDA-DA from Tocris? Please let us know.

Reviews for EDA-DA

There are currently no reviews for this product. Be the first to review EDA-DA and earn rewards!

Have you used EDA-DA?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Fluorescent Probes and Dyes Research Product Guide

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals