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Biological Activity for EDA-DA
EDA-DA is an unnatural dipeptide building block (an ethynyl-D-alanine and a D-alanine). It incorporates a biorthogonal alkyne group into peptidoglycan (PG) through MurF in the cytoplasmic pathway, which enables selective labeling via a click-chemistry reaction. EDA-DA allows labeling of PG in Gram-positive (B. subtilis), Gram-negative (E. coli and C. trachomatis), Mycobacterium (M. smegmatis) and moss plastids (P. patens) with azide modified fluorescent dyes such as Alexa Fluor 488.
Technical Data for EDA-DA
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for EDA-DA
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for EDA-DA
The following data is based on the product molecular weight 298.22. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.35 mL||16.77 mL||33.53 mL|
|5 mM||0.67 mL||3.35 mL||6.71 mL|
|10 mM||0.34 mL||1.68 mL||3.35 mL|
|50 mM||0.07 mL||0.34 mL||0.67 mL|
References for EDA-DA
References are publications that support the biological activity of the product.
Cheung et al (1983) Chloralanyl and propargylglycyl dipeptides. Suicide substrate containing antibacterials. J.Med.Chem. 26 1733 PMID: 6417332
Liechti et al (2013) A new metabolic cell-wall labelling method reveals peptidoglycan in Chlamydia trachomatis. Nature 506 507 PMID: 24336210
Hirano et al (2016) Moss chloroplasts are surrounded by a peptidoglycan wall containing D-amino acids. Plant Cell 28 1521 PMID: 27325639
Kuru et al (2019) Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis. ACS Chem.Biol. 14 2745 PMID: 31743648
Banahene et al (2022) Chemical reporters for bacterial glycans: development and applications. Chem.Rev. 122 3336 PMID: 34905344
If you know of a relevant reference for EDA-DA, please let us know.
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