EB 47

Pricing Availability Delivery Time Qty
Cat.No. 4140 - EB 47 | C24H27N9O6.2HCl | CAS No. 1190332-25-2
Description: Potent PARP-1 inhibitor
Chemical Name: 5'-Deoxy-5'-[4-[2-[(2,3-Dihydro-1-oxo-1H-isoindol-4-yl)amino]-2-oxoethyl]-1-piperazinyl]-5'-oxoadenosine dihydrochloride
Purity: ≥99% (HPLC)
Datasheet
Citations (3)
Literature

Biological Activity

Potent inhibitor of PARP-1 (IC50 = 45 nM). Reduces infarct volume in both a rat transient middle cerebral arterial occlusion model and a cardiac reperfusion model.

Compound Libraries

EB 47 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 610.45
Formula C24H27N9O6.2HCl
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 1190332-25-2
PubChem ID 90488915
InChI Key VVMQSDIMNDTMII-GWZGOYLNSA-N
Smiles O=C(NC2=CC=CC3=C2CNC3=O)CN(CC1)CCN1[C@]([C@@H]4O[C@H](N5C=NC6=C5N=CN=C6N)[C@@H](O)[C@H]4O)=O.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 30.52 50
water 3.05 5mM with gentle warming

Preparing Stock Solutions

The following data is based on the product molecular weight 610.45. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.64 mL 8.19 mL 16.38 mL
5 mM 0.33 mL 1.64 mL 3.28 mL
10 mM 0.16 mL 0.82 mL 1.64 mL
50 mM 0.03 mL 0.16 mL 0.33 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Jagtap et al (2004) The discovery and synthesis of novel adenosine substituted 2,3-dihydro-1H-isoindol-1-ones: potent inhibitors of poly(ADP-ribose) polymerase-1 (PARP-1). Bioorg.Med.Chem.Lett. 14 81 PMID: 14684303

Ferraris et al (2010) Evolution of poly(ADP-ribose) polymerase-1 (PARP-1) inhibitors. From concept to clinic. J.Med.Chem. 53 4561 PMID: 20364863

Gaymes et al (2009) Inhibitors of poly ADP-ribose polymerase (PARP) induce apoptosis of myeloid leukemic cells: potential for therapy of myeloid leukemia and myelodysplastic syndromes. Haematologica 94 638 PMID: 19407318


If you know of a relevant reference for EB 47, please let us know.

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Keywords: EB 47, supplier, EB47, PARP-1, poly(ADP-ribose), polymerase-1, inhibitors, inhibits, PARP1, Poly(ADP-ribose), Polymerase, Poly(ADP-ribose), Polymerase, Tocris Bioscience

3 Citations for EB 47

Citations are publications that use Tocris products. Selected citations for EB 47 include:

Rom et al (2016) PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier Journal of Neuroinflammation 13 254 PMID: 27677851

Rom et al (2015) Poly(ADP-ribose) polymerase-1 inhibition in brain endothelium protects the blood-brain barrier under physiologic and neuroinflammatory conditions. Front Microbiol 35 28 PMID: 25248836

Rom et al (2015) The dual action of poly(ADP-ribose) polymerase -1 (PARP-1) inhibition in HIV-1 infection: HIV-1 LTR inhibition and diminution in Rho GTPase activity. Dev Biol 6 878 PMID: 26379653


Do you know of a great paper that uses EB 47 from Tocris? If so please let us know.

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Literature in this Area

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Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for EB 47

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