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Description: Caged ABT 594 (Cat. No. 6576); suitable for one- or two-photon photolysis
Chemical Name: 5-(((2R)-1-(1-(4,5-Bis(2-(2-(2-methoxyethoxy)ethoxy)ethoxy)-2-nitrophenyl)ethyl)azetidin-2-yl)methoxy)-2-chloropyridine
Purity: ≥98% (HPLC)

Biological Activity for DPNB-ABT 594

DPNB-ABT 594 is a nitrobenzyl-caged ABT 594 (Cat. No. 6576), a selective α4β2 nAChR agonist. One-photon uncaging evokes large inward currents and Ca2+ transients on cell bodies and dendrites of medial habenular neurons in mouse brain slices. Two-photon uncaging induces fast nAChR-mediated currents. Photolyzed with high quantum yield of 0.20. Effective photolysis occurs using one- or two-photon excitation; one-photon uncaging requires illumination at 410 nm for 1.5-3 ms; two-photon uncaging requires illumination with appropriate two photon pulse laser at 710 nm for ~2 ms.

Technical Data for DPNB-ABT 594

M. Wt 672.16
Formula C31H46ClN3O11
Storage Store at -20°C
Purity ≥98% (HPLC)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DPNB-ABT 594

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 67.22 100
ethanol 67.22 100

Preparing Stock Solutions for DPNB-ABT 594

The following data is based on the product molecular weight 672.16. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.49 mL 7.44 mL 14.88 mL
5 mM 0.3 mL 1.49 mL 2.98 mL
10 mM 0.15 mL 0.74 mL 1.49 mL
50 mM 0.03 mL 0.15 mL 0.3 mL

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References for DPNB-ABT 594

References are publications that support the biological activity of the product.

Passlick et al (2018) Optical probing of acetylcholine receptors on neurons in the medial habenula with a novel caged nicotine drug analogue. J.Physiol. 596 5307 PMID: 30222192

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Citations for DPNB-ABT 594

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