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Description: Fluorescent upon binding to Chili aptamer; for imaging RNA in cells
Chemical Name: 4-(4-((Z)-4-Hydroxy-3,5-dimethoxybenzylidene)-2-((hydroxyimino)methyl)-5-oxo-4,5-dihydro-1H-imidazol-1-yl)-N,N,N-trimethylbenzenaminium iodide
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for DMHBO+

DMHBO+ is a cationic fluorophore. Its fluorescence is activated by binding to chili aptamer (Kd = 12 nM). Chili-DMHBO+ complex mimics red fluorescent proteins, suitable for imaging RNA in cells. It is an ideal FRET donor to the rhodamine dye Atto 590 and has applications in FRET-based analytical RNA systems. Excitation/emission maxima (λ) = 456/592 nm; quantum yield = 0.1; stokes shift = 136 nm.

Optical Data for DMHBO+

λabs 456 nm
λem 592 nm
Quantum Yield (φ) 0.1
Application Time-resolved fluorescence energy transfer (TR-FRET)

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Technical Data for DMHBO+

M. Wt 552.37
Formula C22H25IN4O5
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2322286-81-5
Smiles O/N=C/C(N(C1=CC=C(C=C1)[N+](C)(C)C)C/2=O)=NC2=C/C3=CC(OC)=C(C(OC)=C3)O.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DMHBO+

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 27.62 50

Preparing Stock Solutions for DMHBO+

The following data is based on the product molecular weight 552.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.62 mL 18.1 mL 36.21 mL
2.5 mM 0.72 mL 3.62 mL 7.24 mL
5 mM 0.36 mL 1.81 mL 3.62 mL
25 mM 0.07 mL 0.36 mL 0.72 mL

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References for DMHBO+

References are publications that support the biological activity of the product.

Steinmetzger et al (2019) A multicolor large stokes shift fluorogen-activating RNA aptamer with cationic chromophores. Chemistry 25 1931 PMID: 30485561

If you know of a relevant reference for DMHBO+, please let us know.

Keywords: DMHBO+, DMHBO+ supplier, fluorescent, RNA, imaging, red, fluorophore, light-up, aptamers, FLAPs, aptamer-based, probes, Aptamer-based, Imaging, Fluorescent, Probes, 7764, Tocris Bioscience

Citations for DMHBO+

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals