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Cat.No. 6434 - DFHO | C12H9F2N3O3 | CAS No. 1420815-34-4
Description: RFP fluorophore mimic for imaging RNA in living cells; activated by binding Corn aptamers
Chemical Name: 4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity

Mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Corn aptamer (Kd = 70 nM). Excitation/emission maxima (λ) = 505/545 nm; extinction coefficient = 29000 M-1 cm-1.

Technical Data

M. Wt 281.22
Formula C12H9F2N3O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1420815-34-4
Smiles CN1C(/C=N/O)=N/C(C1=O)=C\C2=CC(F)=C(C(F)=C2)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 28.12 100

Preparing Stock Solutions

The following data is based on the product molecular weight 281.22. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.56 mL 17.78 mL 35.56 mL
5 mM 0.71 mL 3.56 mL 7.11 mL
10 mM 0.36 mL 1.78 mL 3.56 mL
50 mM 0.07 mL 0.36 mL 0.71 mL

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References are publications that support the biological activity of the product.

Song et al (2017) Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat.Chem.Biol. 13 1187 PMID: 28945233

Warner et al (2017) A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat.Chem.Biol. 13 1195 PMID: 28945234

If you know of a relevant reference for DFHO, please let us know.

Keywords: DFHO, DFHO supplier, fluorescent, RNA, imaging, fluorophore, red, protein, Corn, aptamer, light, up, aptamers, FLAPs, Aptamers, Aptamer-based, Imaging, Fluorescent, Probes, 6434, Tocris Bioscience

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Literature in this Area

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Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals