DFHO

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Cat.No. 6434 - DFHO | C12H9F2N3O3 | CAS No. 1420815-34-4
Description: RFP fluorophore mimic for imaging RNA in living cells; activated by binding Corn aptamers
Chemical Name: 4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for DFHO

DFHO is a mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. DFHO fluorescence is activated by binding to Corn aptamer (Kd = 70 nM) or Squash aptamer (Kd = 54 nM). Excitation/emission maxima (λ) = 505/545 nm; extinction coefficient = 29000 M-1 cm-1.

Technical Data for DFHO

M. Wt 281.22
Formula C12H9F2N3O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1420815-34-4
Smiles CN1C(/C=N/O)=N/C(C1=O)=C\C2=CC(F)=C(C(F)=C2)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DFHO

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.12 100

Preparing Stock Solutions for DFHO

The following data is based on the product molecular weight 281.22. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.56 mL 17.78 mL 35.56 mL
5 mM 0.71 mL 3.56 mL 7.11 mL
10 mM 0.36 mL 1.78 mL 3.56 mL
50 mM 0.07 mL 0.36 mL 0.71 mL

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References for DFHO

References are publications that support the biological activity of the product.

Song et al (2017) Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat.Chem.Biol. 13 1187 PMID: 28945233

Warner et al (2017) A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat.Chem.Biol. 13 1195 PMID: 28945234

Dey et al (2022) Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 18 180 PMID: 34937909

Truong et al (2022) The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 18 191 PMID: 34937911


If you know of a relevant reference for DFHO, please let us know.

Keywords: DFHO, DFHO supplier, fluorescent, RNA, imaging, fluorophore, red, protein, Corn, aptamer, light, up, aptamers, FLAPs, Aptamers, Aptamer-based, Imaging, Fluorescent, Probes, 6434, Tocris Bioscience

Citations for DFHO

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Literature in this Area

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Fluorescent Probes and Dyes Research Product Guide

Fluorescent Probes and Dyes Research Product Guide

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