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Description: RFP fluorophore mimic for imaging RNA in living cells; activated by binding Corn aptamers
Chemical Name: 4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime
Purity: ≥98% (HPLC)
Citations (1)
Literature (1)

Biological Activity for DFHO

DFHO is a mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. DFHO fluorescence is activated by binding to Corn aptamer (Kd = 70 nM) or Squash aptamer (Kd = 54 nM). Excitation/emission maxima (λ) = 505/545 nm; extinction coefficient = 29000 M-1 cm-1.

Technical Data for DFHO

M. Wt 281.22
Formula C12H9F2N3O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1420815-34-4
Smiles CN1C(/C=N/O)=N/C(C1=O)=C\C2=CC(F)=C(C(F)=C2)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DFHO

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 28.12 100

Preparing Stock Solutions for DFHO

The following data is based on the product molecular weight 281.22. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.56 mL 17.78 mL 35.56 mL
5 mM 0.71 mL 3.56 mL 7.11 mL
10 mM 0.36 mL 1.78 mL 3.56 mL
50 mM 0.07 mL 0.36 mL 0.71 mL

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References for DFHO

References are publications that support the biological activity of the product.

Song et al (2017) Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat.Chem.Biol. 13 1187 PMID: 28945233

Warner et al (2017) A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat.Chem.Biol. 13 1195 PMID: 28945234

Dey et al (2022) Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 18 180 PMID: 34937909

Truong et al (2022) The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 18 191 PMID: 34937911

If you know of a relevant reference for DFHO, please let us know.

Keywords: DFHO, DFHO supplier, fluorescent, RNA, imaging, fluorophore, red, protein, Corn, aptamer, light, up, aptamers, FLAPs, Aptamer-based, Imaging, Fluorescent, Probes, 6434, Tocris Bioscience

1 Citation for DFHO

Citations are publications that use Tocris products. Selected citations for DFHO include:

Aaron E et al (2023) Trumpet is an operating system for simple and robust cell-free biocomputing. Nat Commun 14 2257 PMID: 37080970

Do you know of a great paper that uses DFHO from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals