Decitabine

Pricing Availability Delivery Time Qty
Cat.No. 2624 - Decitabine | C8H12N4O4 | CAS No. 2353-33-5
Description: DNA methyltransferase inhibitor
Alternative Names: 2'-Deoxy-5-azacytidine, 5-Aza-2'-deoxycytidine, NSC 127716
Chemical Name: 4-Amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,3,5-triazin-2(1H)-one
Purity: ≥99% (HPLC)
Datasheet
Citations (4)
Literature

Biological Activity

Cytosine analog that once incorporated into DNA acts as a suicide substrate for DNA methyltransferase. Inhibits DNA methyltransferase and results in DNA hypomethylation and activation of silent genes. Chemotherapeutic agent; suppresses growth of human tumor cell lines. Demethylates differentiation-related genes; reverses embryonic stem cell differentiation.

Compound Libraries

Decitabine is also offered as part of the Tocriscreen Plus, Tocriscreen Epigenetics Toolbox and Tocriscreen Library of FDA-Approved Compounds. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 228.21
Formula C8H12N4O4
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 2353-33-5
PubChem ID 451668
InChI Key XAUDJQYHKZQPEU-KVQBGUIXSA-N
Smiles O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC(N)=NC2=O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.41 50
ethanol 1.14 5
water 11.41 50

Preparing Stock Solutions

The following data is based on the product molecular weight 228.21. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.38 mL 21.91 mL 43.82 mL
5 mM 0.88 mL 4.38 mL 8.76 mL
10 mM 0.44 mL 2.19 mL 4.38 mL
50 mM 0.09 mL 0.44 mL 0.88 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Bender et al (1998) Inhibition of DNA methylation by 5-aza-2'-deoxycytidine supresses the growth of human tumour cell lines. Cancer Res. 58 95 PMID: 9426064

Momparler (2005) Pharmacology of 5-aza-2'-deoxycytidine (decitabine). Semin.Hematol. 42 S9 PMID: 16015507

Jackson-Grusby et al (1997) Mutagenicity of 5-aza-2'-deoxycytidine is mediated by the mammalian DNA methyltransferase. Proc.Natl.Acad.Sci.USA 94 4681 PMID:


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Keywords: Decitabine, supplier, DNA, methyltransferase, inhibitors, inhibits, Transferases, stem, cells, NSC127716, epigenetics, 5-Aza-2'-deoxycytidine, 2'-Deoxy-5-azacytidine, chemotherapeutics, 2'-Deoxy-5-azacytidine, 5-Aza-2'-deoxycytidine, NSC, 127716, DNA, Methyltransferases, DNA, Methyltransferases, Tocris Bioscience

4 Citations for Decitabine

Citations are publications that use Tocris products. Selected citations for Decitabine include:

Wang et al (2012) Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways. PLoS One 7 e39446 PMID: 22761798

Trowbridge et al (2012) Haploinsufficiency of Dnmt1 impairs leukemia stem cell function through derepression of bivalent chromatin domains. BMC Cancer 26 344 PMID: 22345515

Vendetti et al (2015) Evaluation of azacitidine and entinostat as sensitization agents to cytotoxic chemotherapy in preclinical models of non-small cell lung cancer. Genes Dev 6 56 PMID: 25474141

Gu et al (2014) Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy. J Biol Chem 289 14881 PMID: 24695740


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