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Submit ReviewCyanine 7, SE
Description: Near-infrared fluorescent dye for labeling of amines; supplied as NHS ester
Chemical Name: 2-[7-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3,5-heptatrien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95% (HPLC)
Biological Activity
Near-infrared fluorescent dye for labeling of amines. Emission maximum ~764 nm.
Optical Data for Cyanine 7, SE
| Emission Color | Near-IR |
|---|---|
| λabs | 750 nm |
| λem | 764 nm |
| Extinction Coefficient (ε) | 199000 M-1cm-1 |
| Quantum Yield (φ) | 0.3 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.022 |
| Correction Factor 280 | 0.029 |
| Application | Flow Cytometry |
| Alternative For | DyLight 780, Alexa Fluor 750 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
| M. Wt | 779.92 |
| Formula | C39H45N3O10S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 477908-53-5 |
| PubChem ID | 91810959 |
| InChI Key | ROGODJHHEBREAB-UHFFFAOYSA-N |
| Smiles | CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(ON3C(CCC3=O)=O)=O)/C1=C/C=C/C=C/C=C/C4=[N+](CC)C5=C(C=C(S(=O)([O-])=O)C=C5)C4(C)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 77.99 | 100 | |
| DMF | 77.99 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 779.92. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.28 mL | 6.41 mL | 12.82 mL |
| 5 mM | 0.26 mL | 1.28 mL | 2.56 mL |
| 10 mM | 0.13 mL | 0.64 mL | 1.28 mL |
| 50 mM | 0.03 mL | 0.13 mL | 0.26 mL |
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References
References are publications that support the biological activity of the product.
Chen et al (2012) Synthesis and biological evaluation of low molecular weight fluorescent imaging agents for the prostate-specific membrane antigen. Bioconjug.Chem. 23 2377 PMID: 23157641
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Keywords: Cyanine 7, SE, Cyanine 7, SE supplier, Cy7, labeling, amines, fluorescent, imaging, dyes, NHS, ester, Cyanine, Dyes, (Cy, Dyes), Flow, Cytometry, 5437, Tocris Bioscience
Citations for Cyanine 7, SE
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Protocols for Cyanine 7, SE
The following protocol features additional information for the use of Cyanine 7, SE (Cat. No. 5437).
Literature in this Area
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