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Submit ReviewCyanine 5, SE
Description: Red fluorescent dye for the labeling of amines; supplied as NHS ester
Alternative Names: Cy5 NHS Ester
Chemical Name: 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95% (HPLC)
Biological Activity for Cyanine 5, SE
Cyanine 5, SE is a red fluorescent dye for the labeling of amines. Excitation maximum ~649 nm; emission maximum ~666 nm.
For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Optical Data for Cyanine 5, SE
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 649 nm |
| λem | 666 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Closest Laser line | 647 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.03 |
| Correction Factor 280 | 0.04 |
| Application | Fluorescence Microscopy, Confocal Microscopy |
| Alternative For | Alexa Fluor 647; DyLight 649 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Cyanine 5, SE
| M. Wt | 753.88 |
| Formula | C37H43N3O10S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 146368-14-1 |
| PubChem ID | 118705155 |
| InChI Key | WXWLHDCCGVWTDZ-UHFFFAOYSA-N |
| Smiles | CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(ON3C(CCC3=O)=O)=O)/C1=C/C=C/C=C/C4=[N+](CC)C5=C(C=C(S(=O)([O-])=O)C=C5)C4(C)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Cyanine 5, SE
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 75.39 | 100 | |
| DMF | 75.39 | 100 |
Preparing Stock Solutions for Cyanine 5, SE
The following data is based on the product molecular weight 753.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.33 mL | 6.63 mL | 13.26 mL |
| 5 mM | 0.27 mL | 1.33 mL | 2.65 mL |
| 10 mM | 0.13 mL | 0.66 mL | 1.33 mL |
| 50 mM | 0.03 mL | 0.13 mL | 0.27 mL |
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Product Datasheets for Cyanine 5, SE
References for Cyanine 5, SE
References are publications that support the biological activity of the product.
Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407
If you know of a relevant reference for Cyanine 5, SE, please let us know.
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Keywords: Cyanine 5, SE, Cyanine 5, SE supplier, Cyanine, 5SE, red, fluorescent, dyes, amines, labeling, Cy5, NHS, Ester, Dyes, (Cy, Dyes), Flow, Cytometry, 5436, Tocris Bioscience
1 Citation for Cyanine 5, SE
Citations are publications that use Tocris products. Selected citations for Cyanine 5, SE include:
Erik A et al (2022) Directed Evolution of Fluorescent Proteins in Bacteria. Methods Mol Biol 2564 75-97 PMID: 36107338
Do you know of a great paper that uses Cyanine 5, SE from Tocris? Please let us know.
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Protocols for Cyanine 5, SE
The following protocol features additional information for the use of Cyanine 5, SE (Cat. No. 5436).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
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