Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewCyanine 5, SE
Description: Red fluorescent dye (Reactive group: NHS ester). Application: confocal microscopy, fluorescence microscopy, flow cytometry
Alternative Names: Cy5 NHS Ester
Chemical Name: 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95% (HPLC)
Biological Activity for Cyanine 5, SE
Key information: Cyanine 5, SE is a widely used red fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Suitable for fluorescence microscopy, confocal microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 nm and 666 nm, respectively; quantum yield = 0.2; extinction coefficient = 250,000 M-1cm-1; A280 correction factor = 0.04.
For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Optical Data for Cyanine 5, SE
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 649 nm |
| λem | 666 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Closest Laser line | 647 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.03 |
| Correction Factor 280 | 0.04 |
| Application | Fluorescence Microscopy, Confocal Microscopy, Flow cytometry |
| Alternative For | Alexa Fluor® 647; DyLight™ 649 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Cyanine 5, SE
| M. Wt | 753.88 |
| Formula | C37H43N3O10S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 146368-14-1 |
| PubChem ID | 118705155 |
| InChI Key | WXWLHDCCGVWTDZ-UHFFFAOYSA-N |
| Smiles | CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(ON3C(CCC3=O)=O)=O)/C1=C/C=C/C=C/C4=[N+](CC)C5=C(C=C(S(=O)([O-])=O)C=C5)C4(C)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Cyanine 5, SE
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 75.39 | 100 | |
| DMF | 75.39 | 100 |
Preparing Stock Solutions for Cyanine 5, SE
The following data is based on the product molecular weight 753.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.33 mL | 6.63 mL | 13.26 mL |
| 5 mM | 0.27 mL | 1.33 mL | 2.65 mL |
| 10 mM | 0.13 mL | 0.66 mL | 1.33 mL |
| 50 mM | 0.03 mL | 0.13 mL | 0.27 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Cyanine 5, SE
References for Cyanine 5, SE
References are publications that support the biological activity of the product.
Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407
If you know of a relevant reference for Cyanine 5, SE, please let us know.
View Related Products by Target
Keywords: Cyanine 5, SE, Cyanine 5, SE supplier, Cyanine, 5SE, red, fluorescent, dyes, amines, labeling, Cy5, NHS, Ester, Dyes, (Cy, Dyes), Flow, Cytometry, 5436, Tocris Bioscience
1 Citation for Cyanine 5, SE
Citations are publications that use Tocris products. Selected citations for Cyanine 5, SE include:
Erik A et al (2022) Directed Evolution of Fluorescent Proteins in Bacteria. Methods Mol Biol 2564 75-97 PMID: 36107338
Do you know of a great paper that uses Cyanine 5, SE from Tocris? Please let us know.
Reviews for Cyanine 5, SE
There are currently no reviews for this product. Be the first to review Cyanine 5, SE and earn rewards!
Have you used Cyanine 5, SE?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Protocols for Cyanine 5, SE
The following protocol features additional information for the use of Cyanine 5, SE (Cat. No. 5436).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents