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Cyanine 5, SE

Cat. No. 5436 Submit a Review Datasheet / COA / SDS
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Cat.No. 5436 - Cyanine 5, SE | C37H43N3O10S2 | CAS No. 146368-14-1
Description: Red fluorescent dye for the labeling of amines; supplied as NHS ester
Alternative Names: Cy5 NHS Ester
Chemical Name: 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Red fluorescent dye for the labeling of amines. Excitation maximum ~649 nm; emission maximum ~666 nm.

Optical Data for Cyanine 5, SE

Cyanine 5, SE Dye Spectra
Emission Color Red
Brightness
λabs 649 nm
λem 666 nm
Extinction Coefficient (ε) 250000 M-1cm-1
Quantum Yield (φ) 0.2
Closest Laser line 647 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.03
Correction Factor 280 0.04
Alternative For Alexa Fluor 647; DyLight 649

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M. Wt 753.88
Formula C37H43N3O10S2
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 146368-14-1
PubChem ID 118705155
InChI Key WXWLHDCCGVWTDZ-UHFFFAOYSA-N
Smiles CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(ON3C(CCC3=O)=O)=O)/C1=C/C=C/C=C/C4=[N+](CC)C5=C(C=C(S(=O)([O-])=O)C=C5)C4(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 75.39 100
DMF 75.39 100

Preparing Stock Solutions

The following data is based on the product molecular weight 753.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.33 mL 6.63 mL 13.26 mL
5 mM 0.27 mL 1.33 mL 2.65 mL
10 mM 0.13 mL 0.66 mL 1.33 mL
50 mM 0.03 mL 0.13 mL 0.27 mL

Molarity Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the biological activity of the product.

Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407

If you know of a relevant reference for Cyanine 5, SE, please let us know.

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Keywords: Cyanine 5, SE, Cyanine 5, SE supplier, Cyanine, 5SE, red, fluorescent, dyes, amines, labeling, Cy5, NHS, Ester, Dyes, (Cy, Dyes), Flow, Cytometry, 5436, Tocris Bioscience

Citations for Cyanine 5, SE

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Protocols for Cyanine 5, SE

The following protocol features additional information for the use of Cyanine 5, SE (Cat. No. 5436).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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