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Cyanine 3, SE
Orange fluorescent dye for labeling of amines. Emission maximum ~564 nm.
Optical Data for Cyanine 3, SE
|Extinction Coefficient (ε)||150000 M-1cm-1|
|Quantum Yield (φ)||0.31|
|Closest Laser line||532 nm|
|Reactive Group||NHS ester|
|Correction Factor 260||0.04|
|Correction Factor 280||0.09|
|Alternative For||Alexa Fluor 546; DyLight 549|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 727.84. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.37 mL||6.87 mL||13.74 mL|
|5 mM||0.27 mL||1.37 mL||2.75 mL|
|10 mM||0.14 mL||0.69 mL||1.37 mL|
|50 mM||0.03 mL||0.14 mL||0.27 mL|
References are publications that support the biological activity of the product.
Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407
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Keywords: Cyanine 3, SE, Cyanine 3, SE supplier, cyanine, NHS, ester, orange, fluorescent, dye, imaging, amines, labeling, Cy3, Cyanine, Dyes, (Cy, Dyes), Flow, Cytometry, 5435, Tocris Bioscience
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The Sulfo-Cyanine dye was conjugated to bioactive peptide that binds gephyrin. The labeling of gephyrin was done in Cortical neurons (DIV21) infected with lentivirus to express mEos2-Gephyrin.
Protocols for Cyanine 3, SE
The following protocol features additional information for the use of Cyanine 3, SE (Cat. No. 5435).
Literature in this Area
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