Cyanine 3, SE

Discontinued Product

Cyanine 3, SE (Cat. No. 5435) has been withdrawn from sale for commercial reasons.
Cat.No. 5435 - Cyanine 3, SE | C35H41N3O10S2 | CAS No. 146368-16-3
Description: Orange fluorescent dye for labeling of amines; supplied as NHS ester
Chemical Name: 2-[3-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews (1)
Protocols (1)
Literature (1)

Biological Activity for Cyanine 3, SE

Cyanine 3, SE is an orange fluorescent dye for labeling of amines. Emission maximum ~564 nm.

Optical Data for Cyanine 3, SE

Cyanine 3, SE Dye Spectra
Emission Color Orange
Brightness
λabs 550 nm
λem 570 nm
Extinction Coefficient (ε) 150000 M-1cm-1
Quantum Yield (φ) 0.31
Closest Laser line 532 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.04
Correction Factor 280 0.09
Application Flow Cytometry
Alternative For Alexa Fluor 546; DyLight 549

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data for Cyanine 3, SE

M. Wt 727.84
Formula C35H41N3O10S2
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 146368-16-3
PubChem ID 91811030
InChI Key SOVMUUCAZOFIQP-UHFFFAOYSA-N
Smiles CC[N+]1=C(C(C)(C)C2=C1C=CC(S(=O)([O-])=O)=C2)/C=C/C=C3N(CCCCCC(ON4C(CCC4=O)=O)=O)C(C=CC(S(=O)(O)=O)=C5)=C5C/3(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Cyanine 3, SE

References for Cyanine 3, SE

References are publications that support the biological activity of the product.

Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407

Keywords: Cyanine 3, SE, Cyanine 3, SE supplier, cyanine, NHS, ester, orange, fluorescent, dye, imaging, amines, labeling, Cy3, Cyanine, Dyes, (Cy, Dyes), Flow, Cytometry, 5435, Tocris Bioscience

Citations for Cyanine 3, SE

Citations are publications that use Tocris products.

Currently there are no citations for Cyanine 3, SE.

Reviews for Cyanine 3, SE

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Fluorescent probe production and application.
By Vladimir Khayenko on 03/20/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Cortical neurons

The Sulfo-Cyanine dye was conjugated to bioactive peptide that binds gephyrin. The labeling of gephyrin was done in Cortical neurons (DIV21) infected with lentivirus to express mEos2-Gephyrin.

review image

Protocols for Cyanine 3, SE

The following protocol features additional information for the use of Cyanine 3, SE (Cat. No. 5435).

Literature in this Area

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