Cy5B-trisulfo, NHS

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Description: Restricted conformation cyanine dye with high quantum yield and long fluorescence lifetime for advanced microscopy applications
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity for Cy5B-trisulfo, NHS

Key information: Cy5B-trisulfo, NHS is a far-red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Particularly useful for smFRET experiments to reduce blinking and bleaching artefacts and maintain correct mean FRET efficiencies.

Application: FRET, smFRET, FLIM, TIRF, and super resolution microscopy (SRM) techniques, such as dSTORM. Provides enhanced spatial resolution in dSTORM experiments. Does not provide efficient blinking in conventional high-thiol dSTORM buffers; instead, sodium borohydride (NaBH4) addition is required following which SMLM experiments can be performed in PBS.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 685 nm, respectively; extinction coefficient = 241,000 M-1cm-1; quantum yield = 0.4; fluorescence lifetime = 1.85 ns.

We suggest making up a 10 mM stock solution of this product in anhydrous DMSO.

For more information on how Cy5B-trisulfo, NHS may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Licensing Information

Sold under license from the National Institute of Health

Optical Data for Cy5B-trisulfo, NHS

Emission Color Far Red
λabs 669 nm
λem 685 nm
Extinction Coefficient (ε) 241,000 M-1cm-1
Quantum Yield (φ) 0.4
Closest Laser line 640 nm
Reactive Group NHS ester
Reactivity Primary amines
Application SMLM, FRET, smFRET, FLIM, dSTORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Cy5B-trisulfo, NHS

M. Wt 1055.11
Formula C47H52N4Na2O15S3
Storage Store at -20°C
InChI Key WHRLQGVVUOKRRS-IOAHUSCESA-L
Smiles CC1(C)C2=C(C=CC(S(=O)(O[Na])=O)=C2)N(CC[C@@]3([H])C[C@@]4([H])O[C@@]5([H])CC6)C1=C3C=C4C=C5C7=[N+]6C8=C(C=C(S(=O)([O-])=O)C=C8)C7(CCCCC(N(CCC(ON9C(CCC9=O)=O)=O)CCCS(=O)(O[Na])=O)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Cy5B-trisulfo, NHS

References for Cy5B-trisulfo, NHS

References are publications that support the biological activity of the product.

Eiring et al (2021) Targetable conformationally restricted Cyanines enable photon-count-limited applications. Angew.Chem.Int.Ed.Engl. 60 26685 PMID: 34606673

Wäldchen et al (2020) Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM. Nat.Commun. 11 887 PMID: 32060305


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Keywords: Cy5B-trisulfo, NHS, Cy5B-trisulfo, NHS supplier, Cyanine, 5, SE, Cyanine5B, red, fluorescent, fluorescence, dyes, amines, labeling, Cy5, NHS, Ester, SMLM, FRET, smFRET, FLIM, dSTORM, super, resolution, microscopy, amine, reactive, Dyes, (Cy, Dyes), Other, SRM, 7394, Tocris Bioscience

Citations for Cy5B-trisulfo, NHS

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Protocols for Cy5B-trisulfo, NHS

The following protocol features additional information for the use of Cy5B-trisulfo, NHS (Cat. No. 7394).

Literature in this Area

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