CoraFluor™ 2, amine reactive

Pricing Availability   Qty
Description: Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation
Datasheet
Citations
Reviews
Literature (2)

Biological Activity for CoraFluor™ 2, amine reactive

CoraFluor™ 2, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. CoraFluor™ 2 is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. CoraFluor™ 2 is cell permeable and can be used in live cell intracellular TR-FRET assays It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 2 displays red shifted excitation and is excitable by both 365 nm and 405 nm lasers, and is compatible with common acceptor dyes such as FAM/FITC, AF488, GFP and mCherry, enabling seamless assay integration in high throughput screening applications.

CoraFluor 1, amine reactive also available.

Please refer to the protocol for further information on conjugation.

CoraFluor™ is a trademark of Bio-Techne Corp.

Scientific Data

CoraFluor™ 2 stability profile. Stability profiling of CoraFluor™ 2 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca2+, Mg2+, DTT, TCEP = 1 mM; Mn2+, Cu2+ = 0.5 mM).

Binding Activity Live cell TR-FRET assay. View Larger

Live cell TR-FRET assay. Cellular dose-dependent inhibition of HDAC1 via CoraFluor™ 2 labeled HaloTag® in HEK293T cells by various HDAC inhibitors (treatment for 4 h at 37 °C, 1 μM SAHA-NCT). HaloTag is a trademark of Promega Corporation.

Licensing Information

Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Optical Data for CoraFluor™ 2, amine reactive

CoraFluor™ 2, amine reactive Dye Spectra
λabs 352 nm
Reactivity Primary amines
Application Time-resolved fluorescence energy transfer (TR-FRET)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for CoraFluor™ 2, amine reactive

M. Wt 1623.02
Storage Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for CoraFluor™ 2, amine reactive

References for CoraFluor™ 2, amine reactive

References are publications that support the biological activity of the product.

Payne et al (2021) Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 17 1168 PMID: 34675420


If you know of a relevant reference for CoraFluor™ 2, amine reactive, please let us know.

View Related Products by Target

Keywords: CoraFluor™ 2, amine reactive, CoraFluor™ 2, amine reactive supplier, time, resolved, Förster, forster, resonance, energy, transfer, assay, TR, FRET, TRFRET, donor, amine, reactive, probe, conjugation, TR-FRET, and, FP, Assay, Reagents, 7950, Tocris Bioscience

Citations for CoraFluor™ 2, amine reactive

Citations are publications that use Tocris products.

Currently there are no citations for CoraFluor™ 2, amine reactive. Do you know of a great paper that uses CoraFluor™ 2, amine reactive from Tocris? Please let us know.

Reviews for CoraFluor™ 2, amine reactive

There are currently no reviews for this product. Be the first to review CoraFluor™ 2, amine reactive and earn rewards!

Have you used CoraFluor™ 2, amine reactive?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals
Targeted Protein Degradation Research Product Guide

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Ubiquitin-Proteasome System Proteins
  • Assays for Protein Degradation