CC 0651
Biological Activity
Allosteric inhibitor of human Cdc34. Inhibits hCdc34-mediated ubiquitination of p27Kip1 (IC50 = 1.72 μM). Exhibits selectivity for hCdc34 over Uba1, Ube2G1, UbcH7, UbcH5, Ube2N (Ubc13), Ube2R2, SMURF2, SspH1 and Rnf168.
Technical Data
| M. Wt | 442.29 |
| Formula | C20H21Cl2NO6 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1319207-44-7 |
| PubChem ID | 53239927 |
| InChI Key | NTCBTNCWNRCBGX-YTQUADARSA-N |
| Smiles | ClC1=CC(Cl)=CC(C2=CC=C(C[C@H](NC(COC)=O)[C@@H]([C@H](C(O)=O)O)O)C=C2)=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 1eq. NaOH | 44.23 | 100 | |
| DMSO | 44.23 | 100 | |
| ethanol | 22.11 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 442.29. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.26 mL | 11.3 mL | 22.61 mL |
| 5 mM | 0.45 mL | 2.26 mL | 4.52 mL |
| 10 mM | 0.23 mL | 1.13 mL | 2.26 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.45 mL |
Molarity Calculator
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Ceccarelli et al (2011) An allosteric inhibitor of the human Cdc34 ubiquitin-conjugating enzyme. Cell 145 1075 PMID: 21683433
Huang et al (2014) E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Nat.Chem.Biol. 10 156 PMID: 24316736
If you know of a relevant reference for CC 0651, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: CC0651 allosteric inhibitors inhibits hCDC34 ubiquitination selective ubl E2 conjugating enzymes Ubiquitin/Ubl E2 Conjugating Enzymes
Citations for CC 0651
Citations are publications that use Tocris products.
Currently there are no citations for CC 0651. Do you know of a great paper that uses CC 0651 from Tocris? If so please let us know.
