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Cat.No. 2793 - Caffeine | C8H10N4O2 | CAS No. 58-08-2
Description: A1 and A2B antagonist. CNS stimulant
Chemical Name: 3,7-Dihydro-1,3,7-trimethyl-1H-purine-2,6-dione
Purity: ≥99% (HPLC)
Citations (2)

Biological Activity

Central nervous system stimulant. Antagonist at A1 and A2A adenosine receptors and inhibitor of cyclic nucleotide phosphodiesterases. Mobilizes calcium from intracellular stores and inhibits benzodiazepine binding to GABA receptors.

Technical Data

M. Wt 194.19
Formula C8H10N4O2
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 58-08-2
PubChem ID 2519
Smiles O=C1C2=C(N=CN2C)N(C)C(N1C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 9.71 50
ethanol 1.94 10
water 19.42 100

Preparing Stock Solutions

The following data is based on the product molecular weight 194.19. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.15 mL 25.75 mL 51.5 mL
5 mM 1.03 mL 5.15 mL 10.3 mL
10 mM 0.51 mL 2.57 mL 5.15 mL
50 mM 0.1 mL 0.51 mL 1.03 mL

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Product Datasheets

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References are publications that support the products' biological activity.

Nehlig et al (1992) Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects. Brain Res.Brain Res.Rev. 17 139 PMID: 1356551

Fisone et al (2004) Caffeine as a psychomotor stimulant: mechanism of action. Cell.Mol.Life Sci. 61 857 PMID: 15095008

Chen et al (2008) Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease. J.Neuroinflammation 5 12 PMID: 18387175

If you know of a relevant reference for Caffeine, please let us know.

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2 Citations for Caffeine

Citations are publications that use Tocris products. Selected citations for Caffeine include:

Okita et al (2015) Contrasting Effects of the Cytotoxic Anticancer Drug Gemcitabine and the EGFR Tyrosine Kinase Inhibitor Gefitinib on NK Cell-Mediated Cytotoxicity via Regulation of NKG2D Ligand in Non-Small-Cell Lung Cancer Cells. PLoS One 10 e0139809 PMID: 26439264

Seto et al (2013) Acute simvastatin inhibits K ATP channels of porcine coronary artery myocytes. J Cell Sci 8 e66404 PMID: 23799098

Do you know of a great paper that uses Caffeine from Tocris? If so please let us know.

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