Pricing Availability   Qty
Cat.No. 3221 - bPiDDB | C24H38Br2N2 | CAS No. 525596-66-1
Description: Orthosteric neuronal nAChR antagonist
Chemical Name: 1,1'-(1,12-Dodecanediyl)bis[3-methylpyridinium] dibromide
Purity: ≥98% (HPLC)
Citations (1)
Literature (3)

Biological Activity

Orthosteric neuronal nicotinic acetylcholine receptor (nAChR) antagonist (IC50 values are 0.17, 0.25, 0.4, 4.8, 6.5, 8.2, 20 and 34 μM at α3β4, α1β1εδ, α3β4β3, α6β4β3, α7, α4β2, α3β2β3 and α6/3β2β3 receptors respectively). Attenuates nicotine-evoked dopamine release from the ventral tegmental area in vivo (IC50 = 0.2 nM) and reduces nicotine self-administration in rats. Brain penetrant.

Technical Data

M. Wt 514.38
Formula C24H38Br2N2
Storage Desiccate at RT
Purity ≥98% (HPLC)
CAS Number 525596-66-1
PubChem ID 9983961
Smiles CC1=CC=C[N+](CCCCCCCCCCCC[N+]2=CC(C)=CC=C2)=C1.[Br-].[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 51.44 100
DMSO 51.44 100

Preparing Stock Solutions

The following data is based on the product molecular weight 514.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.94 mL 9.72 mL 19.44 mL
5 mM 0.39 mL 1.94 mL 3.89 mL
10 mM 0.19 mL 0.97 mL 1.94 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.


References are publications that support the biological activity of the product.

Dwoskin et al (2008) N,N'alkane-diyl-bis-3-picoliniums as nicotinic receptor antagonists: Inhibition of nicotine-evoked DA release and hyperactivity. J.Pharmacol.Exp.Ther. 326 563 PMID: 18460644

Rahman et al (2008) Region-specific effects of the N,N'-dodecane-1,12-diyl-bis-3-picolinium dibromide on nicotine-induced increase in extracellular DA in vivo. Br.J.Pharmacol. 153 792 PMID: 18059317

Ayers et al (2002) Bis-azaaromatic quaternary ammonium analogues: ligands for α4β2* and β7* subtypes of neuronal nicotinic receptors Bioorg.Med.Chem.Letts. 12 3067

If you know of a relevant reference for bPiDDB, please let us know.

View Related Products by Product Action

View all Nicotinic Receptor (Non-selective) Antagonists

Keywords: bPiDDB, bPiDDB supplier, bPiDDB, nicotinic, acetylcholine, receptors, nachrs, orthosteric, antagonists, a3b4, alpha3beta4, α3β4, brain, penetrant, Nicotinic, Receptors, (Non-selective), 3221, Tocris Bioscience

1 Citation for bPiDDB

Citations are publications that use Tocris products. Selected citations for bPiDDB include:

Holt et al (2015) Pharmacologically distinct nicotinic acetylcholine receptors drive efferent-mediated excitation in calyx-bearing vestibular afferents. J Cell Biol 35 3625 PMID: 25716861

Do you know of a great paper that uses bPiDDB from Tocris? Please let us know.

Reviews for bPiDDB

There are currently no reviews for this product. Be the first to review bPiDDB and earn rewards!

Have you used bPiDDB?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Pain Research Product Guide

A collection of over 280 products for pain research, the guide includes research tools for the study of:

  • Nociception
  • Ion Channels
  • G-Protein-Coupled Receptors
  • Intracellular Signaling
Nicotinic ACh Receptors

Nicotinic ACh Receptors Scientific Review

Updated in 2014, this review by Sue Wonnacott summarizes the diverse structure and function of nicotinic acetylcholine receptors and gives an in-depth review of the ligands available for nAChR research. Compounds available from Tocris are listed.


Pain Poster

Peripheral sensitization is the reduction in the threshold of excitability of sensory neurons that results in an augmented response to a given external stimulus. This poster outlines the excitatory and inhibitory signaling pathways involved in modulation of peripheral sensitization. The role of ion channels, GPCRs, neurotrophins, and cytokines in sensory neurons are also described.