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Description: Nitric oxide activity-based sensing NIR bioluminescent probe
Chemical Name: N-(2-Aminophenyl)-2-((1E,3E)-4-(4-(diethylamino)phenyl)buta-1,3-dien-1-yl)-4,5-dihydrothiazole-4-carboxamide
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for BL660-NO

BL660-NO is an activity-based sensing (ABS) bioluminescent probe for near-infrared red (NIR) detection of nitric oxide (NO). BL660-NO is suitable for in vitro, live cell and deep tissue imaging. Used to visualize endogenous NO in vitro, and in murine models of liver and breast cancer. Used to investigate the effect of diet on tumor microenvironment (TME) in mice.

This product is a scalemic mixture of (S) and (R) enantiomers in which (S) is predominant. The exact ratio is batch specific and is expressed as an enantiomeric excess (e.e.) on the Certificate of Analysis.

Technical Data for BL660-NO

M. Wt 420.58
Formula C24H28N4OS
Storage Store at -20°C
Purity ≥90% (HPLC)
Smiles O=C(NC1=C(N)C=CC=C1)C(CS2)N=C2/C=C/C=C/C(C=C3)=CC=C3N(CC)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BL660-NO

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 42.06 100

Preparing Stock Solutions for BL660-NO

The following data is based on the product molecular weight 420.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.38 mL 11.89 mL 23.78 mL
5 mM 0.48 mL 2.38 mL 4.76 mL
10 mM 0.24 mL 1.19 mL 2.38 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

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References for BL660-NO

References are publications that support the biological activity of the product.

Yadav et al (2022) Activity-based NIR bioluminescence probe enables discovery of diet-induced modulation of the tumor microenvironment via nitric oxide. ACS Cent.Sci. 8 461 PMID: 35505872

If you know of a relevant reference for BL660-NO, please let us know.

Keywords: BL660-NO, BL660-NO supplier, Near, infrared, red, NIR, emission, bioluminescence, deep, tissue, imaging, Nitric, Oxide, NO, in, vivo, cancer, activity, based, sensing, Bioluminescent, Substrates, Other, 7753, Tocris Bioscience

Citations for BL660-NO

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