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Cat.No. 1236 - BHQ | C14H22O2 | CAS No. 88-58-4
Description: Inhibitor of SERCA ATPase
Chemical Name: 1,4-Dihydroxy-2,5-di-tert-butylbenzene
Citations (3)
Reviews (1)

Biological Activity

A selective inhibitor of endoplasmic reticulum Ca2+-ATPase.

Technical Data

M. Wt 222.33
Formula C14H22O2
Storage Store at RT
CAS Number 88-58-4
PubChem ID 2374
Smiles CC(C)(C)C1=CC(O)=C(C=C1O)C(C)(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 100

Preparing Stock Solutions

The following data is based on the product molecular weight 222.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

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Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.5 mL 22.49 mL 44.98 mL
5 mM 0.9 mL 4.5 mL 9 mL
10 mM 0.45 mL 2.25 mL 4.5 mL
50 mM 0.09 mL 0.45 mL 0.9 mL

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References are publications that support the biological activity of the product.

Dettbarn and Palade (1999) Effects of three sarcoplasmic/endoplasmic Ca++ pump inhibitors on release channels and intracellular stores. J.Pharmacol.Exp.Ther. 285 739 PMID: 9580621

Fusi et al (1999) 2,5-Di-t-butyl-1,4 benzohydroquinone induces endothelium-dependent relaxation of rat thoracic aorta. Eur.J.Pharmacol. 366 181 PMID: 10082199

Hassessian et al (1994) Blockade of the inward rectifier potassium current by the Ca2+-ATPase inhibitor 2',5'-di(tert-butyl)-1,4-benzohydroquinone (BHQ). Br.J.Pharmacol. 112 1118 PMID: 7952872

Jan et al (1999) Mechanism of rise and decay of 2,5-di-tert-butylhydroquinone-induced Ca2+ signals in Madin Darby canine kidney cells. Eur.J.Pharmacol. 365 111 PMID: 9988129

If you know of a relevant reference for BHQ, please let us know.

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Keywords: BHQ, BHQ supplier, inhibitors, inhibits, SERCA, ATPase, Ca2+, modulators, Ca2+-ATPase, Signaling, Signalling, Calcium, P-Type, ATPases, Calcium-ATPase, Ion, Transporters, Pumps, 1236, Tocris Bioscience

3 Citations for BHQ

Citations are publications that use Tocris products. Selected citations for BHQ include:

Dai et al (2012) Disturbance of Ca2+ homeostasis converts pro-Met into non-canonical tyrosine kinase p190MetNC in response to endoplasmic reticulum stress in MHCC97 cells. J Biol Chem 287 14586 PMID: 22418436

Zhdanov et al (2011) Bafilomycin A1 activates respiration of neuronal cells via uncoupling associated with flickering depolarization of mitochondria. Cell Mol Life Sci 68 903 PMID: 20820851

Inagaki et al (2014) Pharmacological correction of gating defects in the voltage-gated Ca(v)2.1 Ca2+ channel due to a familial hemiplegic migraine mutation. Neuron 81 91 PMID: 24411734

Do you know of a great paper that uses BHQ from Tocris? Please let us know.

Reviews for BHQ

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BHQ depletes ER Ca2+ store in HEK cells leading to STIM1-Orai1 clustering for store-operated Ca2+ entry..
By Niroj Shrestha on 10/27/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293

ER resident protein, STIM1 and PM resident Orai1 channel were expressed with YFP and CFP fluorescent tags respectively in HEK293 cells and imaged using TIRF microscopy. Basal STIM1 and Orai1 (left) co-clusters to form punctae at PM-ER junctions upon ER store depletion (right) by BHQ (30 µM), leading to store-operated Ca2+ entry through Orai1.

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