BDY FL VH032

Pricing Availability   Qty

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Cat.No. 7483 - BDY FL VH032 | C46H62BF2N7O9S
Description: High-affinity VHL fluorescent probe for TR-FRET and FP assays
Chemical Name: (2S,4R)-1-((S)-2-(tert-Butyl)-21-(5,5-difluoro-7,9-dimethyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-4,19-dioxo-6,9,12,15-tetraoxa-3,18-diazahenicosanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (3)

Biological Activity for BDY FL VH032

BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and theTR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.

Licensing Information

Sold under license from St. Jude Children's Research Hospital

Optical Data for BDY FL VH032

BDY FL VH032 Dye Spectra
Emission Color Green
Brightness
λabs 502 nm
λem 510 nm
Closest Laser line 488 nm
Application TR-FRET and FP assays
Alternative For FAM-DEALAHypYIPMDDDFQLRSF, FAM-DEALA-Hyp-YIPD

Technical Data for BDY FL VH032

M. Wt 937.91
Formula C46H62BF2N7O9S
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key RYFBKCMIPSQUPY-UOIAJAMCSA-N
Smiles O=C(COCCOCCOCCOCCNC(CCC1=CC=C2N1B(F)([N]3=C(C=C(C3=C2)C)C)F)=O)N[C@H](C(N4C[C@@H](C[C@H]4C(NCC5=CC=C(C=C5)C6=C(N=CS6)C)=O)O)=O)C(C)(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BDY FL VH032

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 9.38 10

Preparing Stock Solutions for BDY FL VH032

The following data is based on the product molecular weight 937.91. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 10.66 mL 53.31 mL 106.62 mL
0.5 mM 2.13 mL 10.66 mL 21.32 mL
1 mM 1.07 mL 5.33 mL 10.66 mL
5 mM 0.21 mL 1.07 mL 2.13 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for BDY FL VH032

References are publications that support the biological activity of the product.

Lin et al (2020) Development of BODIPY FL VH032 as a high-affinity and selective von Hippel-Lindau E3 ligase. ACS Omega 6 680 PMID: 33458521


If you know of a relevant reference for BDY FL VH032, please let us know.

Keywords: BDY FL VH032, BDY FL VH032 supplier, selective, high-affinity, VHL, E3, ligase, fluorescent, probe, BODIPY, FL, VH032, TR-FRET, fluorescence, polarization, FP, Enzyme, Probes, and, Substrates, Ubiquitin, Ligases, 7483, Tocris Bioscience

Citations for BDY FL VH032

Citations are publications that use Tocris products.

Currently there are no citations for BDY FL VH032. Do you know of a great paper that uses BDY FL VH032 from Tocris? Please let us know.

Reviews for BDY FL VH032

There are currently no reviews for this product. Be the first to review BDY FL VH032 and earn rewards!

Have you used BDY FL VH032?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Targeted Protein Degradation Research Product Guide

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Custom Degrader Services
  • Ubiquitin-Proteasome System Proteins and Assays
  • Assays for Protein Degradation
Programmed Cell Death Poster

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Targeted Protein Degradation Poster

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia