You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
BDY FL VH032
Biological Activity for BDY FL VH032
BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and the TR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.
Sold under license from St. Jude Children's Research Hospital
Optical Data for BDY FL VH032
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for BDY FL VH032
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BDY FL VH032
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for BDY FL VH032
The following data is based on the product molecular weight 937.91. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||10.66 mL||53.31 mL||106.62 mL|
|0.5 mM||2.13 mL||10.66 mL||21.32 mL|
|1 mM||1.07 mL||5.33 mL||10.66 mL|
|5 mM||0.21 mL||1.07 mL||2.13 mL|
References for BDY FL VH032
References are publications that support the biological activity of the product.
Lin et al (2020) Development of BODIPY FL VH032 as a high-affinity and selective von Hippel-Lindau E3 ligase. ACS Omega 6 680 PMID: 33458521
If you know of a relevant reference for BDY FL VH032, please let us know.
View Related Products by Target
Keywords: BDY FL VH032, BDY FL VH032 supplier, selective, high-affinity, VHL, E3, ligase, fluorescent, probe, BODIPY, FL, VH032, TR-FRET, fluorescence, polarization, FP, Enzyme, Probes, and, Substrates, Ubiquitin, Ligases, Assay, Reagents, 7483, Tocris Bioscience
Citations for BDY FL VH032
Citations are publications that use Tocris products.
Currently there are no citations for BDY FL VH032. Do you know of a great paper that uses BDY FL VH032 from Tocris? Please let us know.
Reviews for BDY FL VH032
There are currently no reviews for this product. Be the first to review BDY FL VH032 and earn rewards!
Have you used BDY FL VH032?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Targeted Protein Degradation Research Product Guide
This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:
- Active Degraders
- TAG Degradation Platform
- Degrader Building Blocks
- Ubiquitin-Proteasome System Proteins
- Assays for Protein Degradation
Programmed Cell Death Poster
There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.
Targeted Protein Degradation Poster
Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia