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BDY FL VH032
Biological Activity for BDY FL VH032
BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and theTR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.
Sold under license from St. Jude Children's Research Hospital
Optical Data for BDY FL VH032
Technical Data for BDY FL VH032
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BDY FL VH032
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for BDY FL VH032
The following data is based on the product molecular weight 937.91. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||10.66 mL||53.31 mL||106.62 mL|
|0.5 mM||2.13 mL||10.66 mL||21.32 mL|
|1 mM||1.07 mL||5.33 mL||10.66 mL|
|5 mM||0.21 mL||1.07 mL||2.13 mL|
References for BDY FL VH032
References are publications that support the biological activity of the product.
Lin et al (2020) Development of BODIPY FL VH032 as a high-affinity and selective von Hippel-Lindau E3 ligase. ACS Omega 6 680 PMID: 33458521
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Citations for BDY FL VH032
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