BDY FL, SE

Pricing Availability   Qty
Cat.No. 5465 - BDY FL, SE | C18H18BF2N3O4 | CAS No. 146616-66-2
Description: Green fluorescent dye for the labeling of amines; supplied as NHS ester
Chemical Name: (T-4)-[1-[3-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrol-2-yl-κN]-1-oxopropoxy]-2,5-pyrrolidinedionato]difluoroboron
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Fluorescent reagent for the labeling of amines. Excitation maximum ~ 502 nm; emission maximum ~ 510 nm.

Optical Data for BDY FL, SE

Emission Color Green
Brightness
λabs 502 nm
λem 510 nm
Extinction Coefficient (ε) 80000 M-1cm-1
Quantum Yield (φ) 0.97
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.015
Correction Factor 280 0.027
Alternative For FAM; Alexa Fluor 488; DyLight 488; Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M. Wt 389.16
Formula C18H18BF2N3O4
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 146616-66-2
PubChem ID 15410442
InChI Key SDVMGNSTOAJONW-UHFFFAOYSA-N
Smiles CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(ON4C(CCC4=O)=O)=O)C=CC3=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.92 100

Preparing Stock Solutions

The following data is based on the product molecular weight 389.16. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.85 mL 25.7 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.28 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References

References are publications that support the biological activity of the product.

Kubohara et al (2013) Mitochondria are the target organelle of differentiation-inducing factor-3, an anti-tumor agent isolated from Dictyostelium discoideum. PLoS ONE 8 e72118 PMID: 23977224


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Keywords: BDY FL, SE, BDY FL, SE supplier, BDYFL,SE, fluorescent, reagents, labeling, amines, bodipy, BDY, Dyes, 5465, Tocris Bioscience

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Literature in this Area

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