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BDY FL, SE
Biological Activity for BDY FL, SE
BDY FL, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to dyes TFAX 488 and FITC. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes. BDY FL also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~504 nM; emission maximum ~510 nM.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY FL, SE
|Extinction Coefficient (ε)||80000 M-1cm-1|
|Quantum Yield (φ)||0.97|
|Closest Laser line||488 nm|
|Reactive Group||NHS ester|
|Correction Factor 260||0.015|
|Correction Factor 280||0.027|
|Application||Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy|
|Alternative For||FAM; Alexa Fluor 488; DyLight 488; Cy2|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for BDY FL, SE
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BDY FL, SE
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for BDY FL, SE
The following data is based on the product molecular weight 389.16. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.57 mL||12.85 mL||25.7 mL|
|5 mM||0.51 mL||2.57 mL||5.14 mL|
|10 mM||0.26 mL||1.28 mL||2.57 mL|
|50 mM||0.05 mL||0.26 mL||0.51 mL|
References for BDY FL, SE
References are publications that support the biological activity of the product.
Kubohara et al (2013) Mitochondria are the target organelle of differentiation-inducing factor-3, an anti-tumor agent isolated from Dictyostelium discoideum. PLoS ONE 8 e72118 PMID: 23977224
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Keywords: BDY FL, SE, BDY FL, SE supplier, BDYFL,SE, fluorescent, reagents, labeling, amines, bodipy, BDY, Dyes, 5465, Tocris Bioscience
Citations for BDY FL, SE
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Protocols for BDY FL, SE
The following protocol features additional information for the use of BDY FL, SE (Cat. No. 5465).
Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
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