BDY FL Thalidomide

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Description: High affinity fluorescent probe for TR-FRET cereblon binding assay
Chemical Name: 3-(5,5-Difluoro-7,9-dimethyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-N-(8-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)octyl)propenamide
Purity: ≥95% (HPLC)
Literature (2)

Biological Activity for BDY FL Thalidomide

BDY FL Thalidomide is a high-affinity fluorescent probe (Kd = 3.6 nM). It can be used in time-resolved fluorescence resonance energy-transfer (TR-FRET) cereblon binding assays for PROTAC® Degrader development. This assay is highly sensitive, selective, and stable. Excitation and emission maxima (λ) are 502 and 510 nm, respectively.

PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Optical Data for BDY FL Thalidomide

BDY FL Thalidomide Dye Spectra
λabs 502 nm
λem 510 nm
Closest Laser line 488 nm
Application Time-resolved fluorescence energy transfer (TR-FRET)
Alternative For FAM; Alexa Fluor 488; DyLight 488; Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data for BDY FL Thalidomide

M. Wt 732.59
Formula C37H43BF2N6O7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2740620-18-0
PubChem ID 165714709
Smiles O=C(CCC1N2C(C3=C(C2=O)C(OCC(NCCCCCCCCNC(CCC4=CC=C5N4[B-](F)([N+]6=C(C=C(C6=C5)C)C)F)=O)=O)=CC=C3)=O)NC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BDY FL Thalidomide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 7.33 10

Preparing Stock Solutions for BDY FL Thalidomide

The following data is based on the product molecular weight 732.59. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 13.65 mL 68.25 mL 136.5 mL
0.5 mM 2.73 mL 13.65 mL 27.3 mL
1 mM 1.37 mL 6.83 mL 13.65 mL
5 mM 0.27 mL 1.37 mL 2.73 mL

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Product Datasheets for BDY FL Thalidomide

References for BDY FL Thalidomide

References are publications that support the biological activity of the product.

Lin et al (2021) General stepwise approach to optimize a TR-FRET assay for characterizing the BRD/PROTAC/CRBN ternary complex. ACS Pharmacol.Transl.Sci. 4 941 PMID: 33860212

Lin et al (2020) Development of BODIPY FL Thalidomide as a high-affinity fluorescent probe for cereblon in a Time-Resolved Fluorescence Resonance Energy Transfer assay. Bioconjug.Chem. 31 2564 PMID: 33070611

If you know of a relevant reference for BDY FL Thalidomide, please let us know.

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Citations for BDY FL Thalidomide

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