You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
AS 99 New
Biological Activity for AS 99
AS 99 is a selective ASH1L histone methyltransferase inhibitor (IC50 = 0.79 μM); it binds to the autoinhibitory loop region in the SET domain (Kd = 0.89 μM). AS 99 exhibits >100-fold selectivity for ASH1L over a range of lysine methyltransferases; no significant inhibition is observed at 50 μM against a panel of 20 histone methyltransferases, including NSD1, NSD2, NSD3, and SETD2. In leukemia cells, AS 99 blocks H3K36 methylation, and causes a dose-dependent downregulation of MLL fusion target genes; it also induces apoptosis and differentiation and inhibits cell proliferation. AS 99 reduces leukemia burden in the xenotransplantation mouse model of MLL leukemia without affecting blood count in normal mice.
Sold under license from the University of Michigan
Technical Data for AS 99
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for AS 99
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for AS 99
The following data is based on the product molecular weight 593.68. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.68 mL||8.42 mL||16.84 mL|
|5 mM||0.34 mL||1.68 mL||3.37 mL|
|10 mM||0.17 mL||0.84 mL||1.68 mL|
|50 mM||0.03 mL||0.17 mL||0.34 mL|
References for AS 99
References are publications that support the biological activity of the product.
Rogawski et al (2021) Discovery of first-in-class inhibitors of ASH1L histone methyltransferase with anti-leukemic activity. Nat.Commun 12 2792 PMID: 33990599
If you know of a relevant reference for AS 99, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: AS 99, AS 99 supplier, AS99, selective, inhibitor, inhibits, inhibitors, ASH1L, histone, methyltransferase, anti-leukemic, leukemia, MLL, H3K36, apoptosis, cell, proliferation, Other, Lysine, Methyltransferases, 7648, Tocris Bioscience
Citations for AS 99
Citations are publications that use Tocris products.
Currently there are no citations for AS 99. Do you know of a great paper that uses AS 99 from Tocris? Please let us know.
Reviews for AS 99
There are currently no reviews for this product. Be the first to review AS 99 and earn rewards!
Have you used AS 99?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image