Ani 9

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Description: Potent and selective TMEM16A (Anoctamin-1; ANO1) blocker
Chemical Name: 2-(4-Chloro-2-methylphenoxy)-N'-(2-methoxybenzylidene)acetohydrazide
Purity: ≥98% (HPLC)
Literature (4)
Pathways (1)

Biological Activity for Ani 9

Ani 9 is a potent and selective TMEM16A (Anoctamin-1; ANO1) blocker (IC50 = 77 nM). Displays no effect on ANO2 activity (at 10 μM). Also exhibits no effect on intracellular calcium signaling or CFTR and ENaC channel activity (at 30 μM).

Technical Data for Ani 9

M. Wt 332.78
Formula C17H17ClN2O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 356102-14-2
PubChem ID 9556542
Smiles COC1=C(C=CC=C1)/C=N/NC(COC2=C(C=C(C=C2)Cl)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Ani 9

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 33.28 100

Preparing Stock Solutions for Ani 9

The following data is based on the product molecular weight 332.78. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3 mL 15.02 mL 30.05 mL
5 mM 0.6 mL 3 mL 6.01 mL
10 mM 0.3 mL 1.5 mL 3 mL
50 mM 0.06 mL 0.3 mL 0.6 mL

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References for Ani 9

References are publications that support the biological activity of the product.

Seo et al (2016) Ani9, a novel potent small-molecule ANO1 inhibitor with negligible effect on ANO2. PLoS One 11 e0155771 PMID: 27219012

If you know of a relevant reference for Ani 9, please let us know.

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Citations for Ani 9

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Literature in this Area

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