Ani 9

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Description: Potent and selective TMEM16A (Anoctamin-1; ANO1) blocker
Chemical Name: 2-(4-Chloro-2-methylphenoxy)-N'-(2-methoxybenzylidene)acetohydrazide
Purity: ≥98% (HPLC)
Citations (2)
Literature (1)
Pathways (1)

Biological Activity for Ani 9

Ani 9 is a potent and selective TMEM16A (Anoctamin-1; ANO1) blocker (IC50 = 77 nM). Displays no effect on ANO2 activity (at 10 μM). Also exhibits no effect on intracellular calcium signaling or CFTR and ENaC channel activity (at 30 μM).

Technical Data for Ani 9

M. Wt 332.78
Formula C17H17ClN2O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 356102-14-2
PubChem ID 9556542
Smiles COC1=C(C=CC=C1)/C=N/NC(COC2=C(C=C(C=C2)Cl)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Ani 9

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 33.28 100

Preparing Stock Solutions for Ani 9

The following data is based on the product molecular weight 332.78. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3 mL 15.02 mL 30.05 mL
5 mM 0.6 mL 3 mL 6.01 mL
10 mM 0.3 mL 1.5 mL 3 mL
50 mM 0.06 mL 0.3 mL 0.6 mL

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References for Ani 9

References are publications that support the biological activity of the product.

Seo et al (2016) Ani9, a novel potent small-molecule ANO1 inhibitor with negligible effect on ANO2. PLoS One 11 e0155771 PMID: 27219012

If you know of a relevant reference for Ani 9, please let us know.

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Keywords: Ani 9, Ani 9 supplier, Ani9, Potent, TMEM16A, Anoctamin-1, blockers, transmembrane, protein, 16A, CaCC, blocks, calcium-activated, chloride, channels, ANO1, Ca2+-Activated, Chloride, Channels, 6076, Tocris Bioscience

2 Citations for Ani 9

Citations are publications that use Tocris products. Selected citations for Ani 9 include:

Dwivedi et al (2023) The TMEM16A blockers benzbromarone and MONNA cause intracellular Ca2+-release in mouse bronchial smooth muscle cells Eur J Pharmacol PMID: 36967079

Heikki et al (2021) ANO1 Expression Orchestrates p27Kip1/MCL1-Mediated Signaling in Head and Neck Squamous Cell Carcinoma. Cancers (Basel) 13 PMID: 33803266

Do you know of a great paper that uses Ani 9 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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Programmed Cell Death Poster

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Ani 9

Apoptosis Signaling Pathway

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.