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AMZ 30 New
Biological Activity for AMZ 30
AMZ 30 is a potent, selective and irreversible inhibitor of protein phosphatase methylesterase-1 (PME-1; IC50 = 600 nM) with > 100-fold selectivity for PME-1 over other serine hydrolases in human cell lysates. AMZ 30 inactivates PME-1 and reduces the demethylated form of serine/threonine protein phosphatase 2A (PP2A) in HEK 293T cells. AMZ 30 perturbs leucine carboxyl methyltransferase-1 (LCMT1)-PME-1 methylation equilibrium leading to shortening of mitotic spindles and mitotic arrest in HeLa cells. It also shows antiproliferative properties in an in vitro model of endometrial carcinoma.
Technical Data for AMZ 30
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for AMZ 30
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for AMZ 30
The following data is based on the product molecular weight 461.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.17 mL||10.84 mL||21.67 mL|
|5 mM||0.43 mL||2.17 mL||4.33 mL|
|10 mM||0.22 mL||1.08 mL||2.17 mL|
|50 mM||0.04 mL||0.22 mL||0.43 mL|
References for AMZ 30
References are publications that support the biological activity of the product.
Elgenaidi et al (2019) Regulation of the phosphoprotein phosphatase 2A system and its modulation during oxidative stress: A potential therapeutic target? Pharmacol.Ther. 198 68 PMID: 30797822
Xia et al (2015) A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size. Cell Cycle 14 1938 PMID: 25839665
Bachovchin et al (2011) Discovery and optimization of sulfonyl acrylonitriles as selective, covalent inhibitors of protein phosphatase methylesterase-1. J.Med.Chem. 54 5229 PMID: 21639134
If you know of a relevant reference for AMZ 30, please let us know.
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Keywords: AMZ 30, AMZ 30 supplier, AMZ30, potent, selective, irreversible, covalent, inhibitor, inhibits, serine, hydrolases, protein, phosphatase, methylesterase-1, PME-1, mitotic, arrest, Other, Hydrolases, Mitosis, 6923, Tocris Bioscience
Citations for AMZ 30
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