Ampicillin sodium salt

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Cat.No. 5503 - Ampicillin sodium salt | C16H18N3NaO4S | CAS No. 69-52-3
Description: Antibiotic; selection reagent
Chemical Name: (2S,5R,6R)-6-[[(2R)-2-Amino-2-phenylacetyl)amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid sodium salt
Reviews (1)
Literature (1)

Biological Activity

Antibiotic. Widely used selection reagent for transformed cells expressing β-lactamase.

Technical Data

M. Wt 371.39
Formula C16H18N3NaO4S
Storage Store at +4°C
CAS Number 69-52-3
PubChem ID 23663979
Smiles N[C@@H](C(N[C@@H]2C(N3[C@@]([H])2SC(C)(C)[C@@H]3[C@](O[Na])=O)=O)=O)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 37.14 100

Preparing Stock Solutions

The following data is based on the product molecular weight 371.39. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.69 mL 13.46 mL 26.93 mL
5 mM 0.54 mL 2.69 mL 5.39 mL
10 mM 0.27 mL 1.35 mL 2.69 mL
50 mM 0.05 mL 0.27 mL 0.54 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Keywords: Ampicillin sodium salt, Ampicillin sodium salt supplier, antibiotics, cell, selection, beta-lactamase, b-lactamase, βlactamase, Antibiotics, Biochemicals, and, Molecular, Biology, 5503, Tocris Bioscience

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Insertional inactivation of an antibiotic resistance gene.
By Anonymous on 11/15/2018
Assay Type: In Vitro
Species: Human

Blue/white screening (Insertional inactivation of an enzyme coding gene) was carried out.The vector molecule contains genes that when expressed confer resistance to the antibiotics ampicillin.Bacteria (E. coli, lacZ’-) containing non-recombinant plasmid grows on ampicillin plates (due to the ampicillin resistance gene) and have a blue colour (in the presence of X-gal) due to the presence of the lacZ’ gene.

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