Acridine Orange hydrochloride
Cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double stranded DNA and red fluorescence when bound to RNA or single stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 301.81. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.31 mL||16.57 mL||33.13 mL|
|5 mM||0.66 mL||3.31 mL||6.63 mL|
|10 mM||0.33 mL||1.66 mL||3.31 mL|
|50 mM||0.07 mL||0.33 mL||0.66 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Kiyoshima et al (2013) Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 8 80998 PMID: 24278362
McMaster et al (1977) Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 74 4835 PMID: 73185
Ratan et al (2008) Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 62 376 PMID: 7903353
If you know of a relevant reference for Acridine Orange hydrochloride, please let us know.
Keywords: nucleic acid binding dyes cell cycle lysosomal DNA stains RNA Fluorescent Dyes
Citations for Acridine Orange hydrochloride
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Literature in this Area
New Product Guide (Spring/Summer 2017)New
Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.
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