Acridine Orange hydrochloride

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Cat.No. 5092 - Acridine Orange hydrochloride | C17H19N3.HCl | CAS No. 65-61-2
Description: Nucleic acid binding dye
Chemical Name: N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
Purity: ≥99% (HPLC)
Literature (1)

Biological Activity for Acridine Orange hydrochloride

Acridine Orange hydrochloride is a cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double-stranded DNA and red fluorescence when bound to RNA or single-stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.

Technical Data for Acridine Orange hydrochloride

M. Wt 301.81
Formula C17H19N3.HCl
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 65-61-2
PubChem ID 517204
Smiles CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Acridine Orange hydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 30.18 100
DMSO 30.18 100

Preparing Stock Solutions for Acridine Orange hydrochloride

The following data is based on the product molecular weight 301.81. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.31 mL 16.57 mL 33.13 mL
5 mM 0.66 mL 3.31 mL 6.63 mL
10 mM 0.33 mL 1.66 mL 3.31 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

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Product Datasheets for Acridine Orange hydrochloride

Certificate of Analysis / Product Datasheet
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References for Acridine Orange hydrochloride

References are publications that support the biological activity of the product.

Kiyoshima et al (2013) Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 8 80998 PMID: 24278362

McMaster et al (1977) Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 74 4835 PMID: 73185

Ratan et al (2008) Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 62 376 PMID: 7903353

If you know of a relevant reference for Acridine Orange hydrochloride, please let us know.

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Citations for Acridine Orange hydrochloride

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