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Acridine Orange hydrochloride
Cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double-stranded DNA and red fluorescence when bound to RNA or single-stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 301.81. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.31 mL||16.57 mL||33.13 mL|
|5 mM||0.66 mL||3.31 mL||6.63 mL|
|10 mM||0.33 mL||1.66 mL||3.31 mL|
|50 mM||0.07 mL||0.33 mL||0.66 mL|
References are publications that support the biological activity of the product.
Kiyoshima et al (2013) Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 8 80998 PMID: 24278362
McMaster et al (1977) Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 74 4835 PMID: 73185
Ratan et al (2008) Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 62 376 PMID: 7903353
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