Acridine Orange hydrochloride

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Cat.No. 5092 - Acridine Orange hydrochloride | C17H19N3.HCl | CAS No. 65-61-2
Description: Nucleic acid binding dye
Chemical Name: N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
Purity: ≥99% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double stranded DNA and red fluorescence when bound to RNA or single stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.

Technical Data

M. Wt 301.81
Formula C17H19N3.HCl
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 65-61-2
PubChem ID 517204
InChI Key VSTHNGLPHBTRMB-UHFFFAOYSA-N
Smiles CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 30.18 100
DMSO 30.18 100

Preparing Stock Solutions

The following data is based on the product molecular weight 301.81. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.31 mL 16.57 mL 33.13 mL
5 mM 0.66 mL 3.31 mL 6.63 mL
10 mM 0.33 mL 1.66 mL 3.31 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Kiyoshima et al (2013) Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 8 80998 PMID: 24278362

McMaster et al (1977) Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 74 4835 PMID: 73185

Ratan et al (2008) Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 62 376 PMID: 7903353


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