Acridine Orange hydrochloride

Pricing Availability   Qty
Description: Nucleic acid binding dye
Chemical Name: N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for Acridine Orange hydrochloride

Acridine Orange hydrochloride is a cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double-stranded DNA and red fluorescence when bound to RNA or single-stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.

This product is supplied as a hydrochloride salt, the stoichiometry of which is batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Technical Data for Acridine Orange hydrochloride

M. Wt 265.36 (Free Base)
Formula C17H19N3.HCl
Storage Store at RT
Purity ≥98% (HPLC)
PubChem ID 517204
InChI Key VSTHNGLPHBTRMB-UHFFFAOYSA-N
Smiles CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Acridine Orange hydrochloride

Solubility Soluble to 10 mg/ml in water

Product Datasheets for Acridine Orange hydrochloride

Certificate of Analysis / Product Datasheet
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References for Acridine Orange hydrochloride

References are publications that support the biological activity of the product.

Kiyoshima et al (2013) Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 8 80998 PMID: 24278362

McMaster et al (1977) Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 74 4835 PMID: 73185

Ratan et al (2008) Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 62 376 PMID: 7903353


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Keywords: Acridine Orange hydrochloride, Acridine Orange hydrochloride supplier, nucleic, acid, binding, dyes, cell, cycle, lysosomal, DNA, stains, RNA, 65612, 65-61-2, Fluorescent, Stains, Probes, Cell, Viability, and, Dyes, 5092, Tocris Bioscience

Citations for Acridine Orange hydrochloride

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals