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Submit ReviewAcridine Orange hydrochloride
Description: Nucleic acid binding dye. Cell permeable. Used for: cell cycle and apoptosis determination. Application: flow cytometry, fluorescence microscopy
Chemical Name: N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
Purity: ≥98% (HPLC)
Biological Activity for Acridine Orange hydrochloride
Key information: Acridine Orange hydrochloride is a nucleic acid binding dye. Cell and organelle permeable.
Used for: cell cycle and apoptosis determination, for visual detection of nucleic acids on agarose and polyacrylamide gels, for autophagy analyze.
Application: suitable for flow cytometry and fluorescence microscopy.
Properties and Photophysical Data: emits green fluorescence when incorporated into double-stranded DNA, red fluorescence when bound electrostatically to phosphate groups of RNA or single-stranded DNA, and orange fluorescence in acidic conditions. Excitation and emission maxima (λ) are 502 nm and 525 nm, respectively (green, double strands)/ 460 nm and 650 nm, respectively (red, single strands)/ and 475 nm and 590 nm, respectively (orange, acidic conditions).
This product is supplied as a hydrochloride salt, the stoichiometry of which is batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Technical Data for Acridine Orange hydrochloride
| M. Wt | 265.36 (Free Base) |
| Formula | C17H19N3.HCl |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| PubChem ID | 517204 |
| InChI Key | VSTHNGLPHBTRMB-UHFFFAOYSA-N |
| Smiles | CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Acridine Orange hydrochloride
| Solubility | Soluble to 10 mg/ml in water |
Product Datasheets for Acridine Orange hydrochloride
References for Acridine Orange hydrochloride
References are publications that support the biological activity of the product.
Kiyoshima et al (2013) Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 8 80998 PMID: 24278362
McMaster et al (1977) Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 74 4835 PMID: 73185
Ratan et al (2008) Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 62 376 PMID: 7903353
Sato et al (2018) Flow cytometric analysis of Xenopus laevis and X. tropicalis blood cells using acridine orange. Sci.Rep. 8 16245 PMID: 30390005
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1 Citation for Acridine Orange hydrochloride
Citations are publications that use Tocris products. Selected citations for Acridine Orange hydrochloride include:
Stephan et al (2022) Imatinib disturbs lysosomal function and morphology and impairs the activity of mTORC1 in human hepatocyte cell lines. Food Chem Toxicol 162 112869 PMID: 35182693
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
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