Ac-LEHD-AFC

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Cat.No. 1575 - Ac-LEHD-AFC | N-Ac-Leu-Glu-His-Asp-(7-amino-4-trifluoromethylcoumarin) | CAS No. 210345-03-2
Description: Fluorogenic caspase substrate
Chemical Name: N-Acetyl-Leu-Glu-His-Asp-(7-amino-4-trifluoromethylcoumarin)
Datasheet
Citations
Literature

Biological Activity

Fluorogenic caspase substrate. Analog of the caspase-9 substrate, LEHD-AFC.

Technical Data

M. Wt 765.7
Formula C33H38F3N7O11
Sequence LEHD

(Modifications: Leu-1 = N-Ac-Leu, Asp-4 = Asp-(7-amino-4-trifluoromethylcoumarin))

Storage Desiccate at -20°C
CAS Number 210345-03-2
PubChem ID 25108576
InChI Key HULKIXRFKRCRHD-ZJZGAYNASA-N
Smiles CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(O)=O)C(=O)NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 1 mg/ml in water

Preparing Stock Solutions

The following data is based on the product molecular weight 765.7. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.31 mL 6.53 mL 13.06 mL
5 mM 0.26 mL 1.31 mL 2.61 mL
10 mM 0.13 mL 0.65 mL 1.31 mL
50 mM 0.03 mL 0.13 mL 0.26 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Ito et al (1999) Possible involvement of cytochrome c release and sequential activation of caspases in ceramide-induced apoptosis in SK-N-MC cells. Biochim.Biophys.Acta 1452 263 PMID: 10590315

Moriya et al (2000) Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484 253 PMID: 11078888


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Keywords: Ac-LEHD-AFC, supplier, Fluorogenic, caspases, Proteinases, Proteases, Caspases, Caspases, Tocris Bioscience

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