Fluorogenic caspase substrate. Analog of the caspase-9 substrate, LEHD-AFC.
(Modifications: Leu-1 = N-Ac-Leu, Asp-4 = Asp-(7-amino-4-trifluoromethylcoumarin))
|Storage||Desiccate at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solubility||Soluble to 1 mg/ml in water|
Preparing Stock Solutions
The following data is based on the product molecular weight 765.7. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.31 mL||6.53 mL||13.06 mL|
|5 mM||0.26 mL||1.31 mL||2.61 mL|
|10 mM||0.13 mL||0.65 mL||1.31 mL|
|50 mM||0.03 mL||0.13 mL||0.26 mL|
References are publications that support the products' biological activity.
Ito et al (1999) Possible involvement of cytochrome c release and sequential activation of caspases in ceramide-induced apoptosis in SK-N-MC cells. Biochim.Biophys.Acta 1452 263 PMID: 10590315
Moriya et al (2000) Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484 253 PMID: 11078888
If you know of a relevant reference for Ac-LEHD-AFC, please let us know.
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Keywords: Ac-LEHD-AFC, supplier, Fluorogenic, caspases, Proteinases, Proteases, Caspases, Caspases, Tocris Bioscience
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