Peptide sequence shown to be preferred recognition motif for the serine protease granzyme B. Fluorogenic substrate.
(Modifications: Ile-1 = N-Ac-Ile, Asp-4 = Asp-(7-amino-4-trifluoromethylcoumarin))
|Storage||Desiccate at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solubility||Soluble to 14.51 mg/ml in DMSO|
Preparing Stock Solutions
The following data is based on the product molecular weight 725.67. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.38 mL||6.89 mL||13.78 mL|
|5 mM||0.28 mL||1.38 mL||2.76 mL|
|10 mM||0.14 mL||0.69 mL||1.38 mL|
|50 mM||0.03 mL||0.14 mL||0.28 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Thornberry et al (1997) A combinatorial approach defines specificities of members of the caspase family and granzyme B. J.Biol.Chem. 272 17907 PMID: 9218414
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Keywords: Fluorogenic granzyme B Caspases Proteinases Proteases Caspases
Citations for Ac-IEPD-AFC
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