A 196

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Cat.No. 6167 - A 196 | C18H16Cl2N4 | CAS No. 1982372-88-2
Description: Potent and selective SUV420H1/H2 inhibitor
Chemical Name: 6,7-Dichloro-N-cyclopentyl-4-(4-pyridinyl)-1-phthalazinamine
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Potent and selective SUV420H1/H2 inhibitor (IC50 values are 25 and 144 nM respectively). Exhibits 100-fold selectivity over other histone methyltransferases and non-epigenetic targets. Inhibits the di- and tri-methylation of H4K20me in multiple cell lines (IC50 < 1 μM).

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the A-196 probe summary on the SGC website.

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of A196 is reviewed on the Chemical Probes website.

Technical Data

M. Wt 359.25
Formula C18H16Cl2N4
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1982372-88-2
Smiles ClC1=CC2=C(C(C3=CC=NC=C3)=NN=C2NC4CCCC4)C=C1Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.18 20

Preparing Stock Solutions

The following data is based on the product molecular weight 359.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.78 mL 13.92 mL 27.84 mL
5 mM 0.56 mL 2.78 mL 5.57 mL
10 mM 0.28 mL 1.39 mL 2.78 mL
50 mM 0.06 mL 0.28 mL 0.56 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Bromberg et al (2017) The SUV4-20 inhibitor A-196 verifies a role for epigenetics in genomic integrity. Nat.Chem.Biol. 13 317 PMID: 28114273


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Citations for A 196

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Epigenetics

Epigenetics Scientific Review

Written by Susanne Müller-Knapp and Peter J. Brown, this review gives an overview of the development of chemical probes for epigenetic targets, as well as the impact of these tool compounds being made available to the scientific community. In addition, their biological effects are also discussed. Epigenetic compounds available from Tocris are listed.

Pathways for A 196

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