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Submit Review5-TAMRA NHS is an orange-fluorescent dye that is commonly used for labeling proteins, peptides and oligonucleotides containing primary amines. Excitation maximum = 546 nm; emission maximum = 580 nm
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to prepare stock solutions in DMSO.
Emission Color | Orange |
---|---|
Brightness | |
λabs | 546 nm |
λem | 580 nm |
Extinction Coefficient (ε) | 95,000 M-1cm-1 |
Quantum Yield (φ) | 0.1 |
Closest Laser line | 532 nm |
Reactive Group | NHS ester |
Reactivity | Primary amines |
Cell Permeable | Yes |
pH Sensitivity | No |
Correction Factor 280 | 0.178 |
Application | Fluorescence Microscopy, Confocal Microscopy |
Alternative For | Alexa Fluor® 546 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral ViewerM. Wt | 527.52 |
Formula | C29H25N3O7 |
Storage | Store at -20°C |
Purity | ≥90% (HPLC) |
CAS Number | 150810-68-7 |
PubChem ID | 2762600 |
InChI Key | VWFRSNKRTNUMET-UHFFFAOYSA-N |
Smiles | CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
References are publications that support the biological activity of the product.
Kvach et al (2009) Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug.Chem. 20 1673 PMID: 19606815
Hirata et al (2014) Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 136 11546 PMID: 25036716
If you know of a relevant reference for 5-TAMRA NHS, please let us know.
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The following protocol features additional information for the use of 5-TAMRA NHS (Cat. No. 7665).
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: