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Description: Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides
Chemical Name: 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
Purity: ≥90% (HPLC)
Biological Activity for 5-TAMRA NHS
5-TAMRA NHS is an orange-fluorescent dye that is commonly used for labeling proteins, peptides and oligonucleotides containing primary amines. Excitation maximum = 546 nm; emission maximum = 580 nm
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to prepare stock solutions in DMSO.
Optical Data for 5-TAMRA NHS
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 546 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95,000 M-1cm-1 |
| Quantum Yield (φ) | 0.1 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| pH Sensitivity | No |
| Correction Factor 280 | 0.178 |
| Application | Fluorescence Microscopy, Confocal Microscopy |
| Alternative For | Alexa Fluor® 546 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for 5-TAMRA NHS
| M. Wt | 527.52 |
| Formula | C29H25N3O7 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 150810-68-7 |
| PubChem ID | 2762600 |
| InChI Key | VWFRSNKRTNUMET-UHFFFAOYSA-N |
| Smiles | CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for 5-TAMRA NHS
References for 5-TAMRA NHS
References are publications that support the biological activity of the product.
Kvach et al (2009) Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug.Chem. 20 1673 PMID: 19606815
Hirata et al (2014) Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 136 11546 PMID: 25036716
If you know of a relevant reference for 5-TAMRA NHS, please let us know.
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Protocols for 5-TAMRA NHS
The following protocol features additional information for the use of 5-TAMRA NHS (Cat. No. 7665).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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