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Description: Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides
Chemical Name: 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
Purity: ≥90% (HPLC)
Protocols (1)
Literature (1)

Biological Activity for 5-TAMRA NHS

5-TAMRA NHS is an orange-fluorescent dye that is commonly used for labeling proteins, peptides and oligonucleotides containing primary amines. Excitation maximum = 546 nm; emission maximum = 580 nm

Please see the protocol for further information on protein/antibody labeling and conjugation.

It is recommended to prepare stock solutions in DMSO.

Optical Data for 5-TAMRA NHS

Emission Color Orange
λabs 546 nm
λem 580 nm
Extinction Coefficient (ε) 95,000 M-1cm-1
Quantum Yield (φ) 0.1
Closest Laser line 532 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
pH Sensitivity No
Correction Factor 280 0.178
Application Fluorescence Microscopy, Confocal Microscopy
Alternative For Alexa Fluor® 546

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for 5-TAMRA NHS

M. Wt 527.52
Formula C29H25N3O7
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 150810-68-7
PubChem ID 2762600
Smiles CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

References for 5-TAMRA NHS

References are publications that support the biological activity of the product.

Kvach et al (2009) Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug.Chem. 20 1673 PMID: 19606815

Hirata et al (2014) Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 136 11546 PMID: 25036716

If you know of a relevant reference for 5-TAMRA NHS, please let us know.

Keywords: 5-TAMRA, NHS, 5-TAMRA, NHS supplier, 5TAMRANHS, 5tamrase, fluorescent, dyes, DNA, RNA, amine, reactive, peptides, oligonucleotides, proteins, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, 7665, Tocris Bioscience

Citations for 5-TAMRA NHS

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Protocols for 5-TAMRA NHS

The following protocol features additional information for the use of 5-TAMRA NHS (Cat. No. 7665).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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