1400W dihydrochloride

Pricing Availability   Qty
Description: Potent, highly selective iNOS inhibitor
Chemical Name: N-[[3-(Aminomethyl)phenyl]methyl]-ethanimidamide dihydrochloride
Purity: ≥99% (HPLC)
Citations (9)
Reviews (3)
Literature (1)

Biological Activity for 1400W dihydrochloride

1400W dihydrochloride is a slow, tight binding, potent and highly selective inhibitor of inducible nitric oxide synthase (Kd = 7 nM). Selective over nNOS and eNOS (Ki values are 2 and 50 μM respectively). Cell-permeable and active in vivo. Neuroprotective in epilepsy models and has analgesic effects in models of mechanical and heat hypersensitivity.

Technical Data for 1400W dihydrochloride

M. Wt 250.17
Formula C10H15N3.2HCl
Storage Desiccate at RT
Purity ≥99% (HPLC)
CAS Number 214358-33-5
PubChem ID 2733515
Smiles Cl.Cl.CC(=N)NCC1=CC=CC(CN)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 1400W dihydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 25.02 100

Preparing Stock Solutions for 1400W dihydrochloride

The following data is based on the product molecular weight 250.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4 mL 19.99 mL 39.97 mL
5 mM 0.8 mL 4 mL 7.99 mL
10 mM 0.4 mL 2 mL 4 mL
50 mM 0.08 mL 0.4 mL 0.8 mL

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Product Datasheets for 1400W dihydrochloride

Certificate of Analysis / Product Datasheet
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References for 1400W dihydrochloride

References are publications that support the biological activity of the product.

Garvey et al (1997) 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J.Biol.Chem. 272 4959 PMID: 9030556

Parmentier et al (1999) Selective inhibition of inducible nitric oxide synthase prevents ischaemic brain injury. Br.J.Pharmacol. 127 546 PMID: 10385257

Thomsen et al (1997) Selective inhibition of inducible nitric oxide inhibits tumor growth in vivo: studies with 1400W, a novel inhibitor. Cancer Res. 57 3300 PMID: 9242464

Staunton et al (2018) Inducible nitric oxide synthase inhibition by 1400W limits pain hypersensitivity in a neuropathic pain rat model. Exp.Physiol 103 535 PMID: 29441689

Putra et al (2020) Inducible nitric oxide synthase inhibitor, 1400W, mitigates DFP-induced long-term neurotoxicity in the rat model. Neurobiol.Dis. 133 104443 PMID: 30940499

If you know of a relevant reference for 1400W dihydrochloride, please let us know.

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Keywords: 1400W dihydrochloride, 1400W dihydrochloride supplier, Potent, selective, iNOS, inhibitors, inhibits, Nitric, Oxide, Signaling, Signalling, Inducible, NO, Synthases, 1415, Tocris Bioscience

9 Citations for 1400W dihydrochloride

Citations are publications that use Tocris products. Selected citations for 1400W dihydrochloride include:

Müller et al (2018) Both Type I and Type II Interferons Can Activate Antitumor M1 Macrophages When Combined With TLR Stimulation. Front Immunol 9 2520 PMID: 30450098

Gresa-Arribas et al (2012) Modelling neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents. PLoS One 7 e45227 PMID: 23028862

Tsuda et al (2009) Mechanisms underlying fibronectin-induced up-regulation of P2X4R expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signalling pathways. J Cell Mol Med 13 3251 PMID: 19298529

Clark et al (2015) Selective activation of microglia facilitates synaptic strength. RNA 35 4552 PMID: 25788673

Do you know of a great paper that uses 1400W dihydrochloride from Tocris? Please let us know.

Reviews for 1400W dihydrochloride

Average Rating: 5 (Based on 3 Reviews.)

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Inhibition of iNOS by 1400W.
By Anonymous on 04/03/2021
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: primary BMDM

Macrophages were treated with LPS (10 ng/mL) and IFN-y (20 ng/mL) for 24h in the presence of 1400W (100 uM)

review image

1400W inhibits iNOS expression induced by LPS.
By Anonymous on 05/16/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Macrophages

RAW macrophages were treated with 200 ng/ml LPS alone or after pre-treatment with 1 μM 1400W (30 min pre-incubation) for 8 h to follow iNOS expression using Western blot. LPS treatment elevated iNOS expression while 1400W pretreatment significantly blunted this effect.

review image

iNOS inhibitor.
By Anonymous on 07/28/2018
Assay Type: Ex Vivo
Species: Mouse

Used as iNOS inhibitor for organ bath experiments with rat and mouse aorta

Good solubility and stability

Literature in this Area

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Cancer Research Product Guide

Cancer Research Product Guide

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