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Submit ReviewKyoto Probe-1
说明： Fluorescent probe that selectively identifies undifferentiated iPS/ES cells
别名： KP1
化学名： 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate
纯度： ≥95% (HPLC)
生物活性 for Kyoto Probe-1
Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.
Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.
Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45
许可信息
Sold under license from New York University
Optical Data for Kyoto Probe-1
| Emission Color | Green |
|---|---|
| λabs | 515 nm |
| λem | 529 nm |
| Quantum Yield (φ) | 0.45 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Live-cell Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for Kyoto Probe-1
| 分子量 | 418.35 |
| 公式 | C19H13FN2O.CF3CO2H |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 2088021-81-0 |
| InChI Key | ADFLZJALCLDWDM-UHFFFAOYSA-N |
| Smiles | [O-]C(C(F)(F)F)=O.[NH2+]=C1C=C2OC3=C(C(C4=CC=C(C=C4)F)=C2C=C1)C=CC(N)=C3 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for Kyoto Probe-1
参考文献 for Kyoto Probe-1
参考文献是支持产品生物活性的出版物。
Mao et al (2017) A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures. Angew.Chem.Int.Ed.Engl. 56 1765 PMID: 28067441
Hirata et al (2014) A chemical probe that labels human pluripotent stem cells. Cell Rep. 6 1165 PMID: 24613351
Miyagi-Shiohira et al (2020) Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells. Sci.Rep. 10 18084 PMID: 33093580
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该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
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- Differentiation
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- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.