Yoda 1

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Description: Piezo1 channel activator
Chemical Name: 2-[5-[[(2,6-Dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]pyrazine
Purity: ≥99% (HPLC)
Datasheet
Citations (38)
Reviews (2)

Biological Activity for Yoda 1

Yoda 1 is a selective activator of mouse and human mechanosensitive channel piezo1. In HEK cells, Yoda 1 slows the inactivation phase of transient currents, sensitizes Piezo1 to activation by pressure, and partially activates channels in the absence of external pressure. In MDCK cells, Yoda 1 induces Ca2+-dependent chromatin hypercondensation and alters Ca2+-dependent myosin contractility, leading to nuclear shrinkage in cells. In red blood cells from sickle cell anemia (SCA) patients, Yoda 1 increases intracellular Ca2+ and phosphatidylserine exposure, which leads to KCa3.1 channel mediated Ca2+ influx and K+ and water efflux, causing shrinkage of the cells.

Compound Libraries for Yoda 1

Yoda 1 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Yoda 1

M. Wt 355.27
Formula C13H8Cl2N4S2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 448947-81-7
PubChem ID 2746822
InChI Key BQNXBSYSQXSXPT-UHFFFAOYSA-N
Smiles ClC1=C(CSC2=NN=C(C3=NC=CN=C3)S2)C(Cl)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Yoda 1

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.11 20

Preparing Stock Solutions for Yoda 1

The following data is based on the product molecular weight 355.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 14.07 mL 70.37 mL 140.74 mL
1 mM 2.81 mL 14.07 mL 28.15 mL
2 mM 1.41 mL 7.04 mL 14.07 mL
10 mM 0.28 mL 1.41 mL 2.81 mL

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Product Datasheets for Yoda 1

Certificate of Analysis / Product Datasheet
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References for Yoda 1

References are publications that support the biological activity of the product.

Syeda et al (2015) Chemical activation of the mechanotransduction channel Piezo1. ELife 4 e07369 PMID: 26001275

Mikhailov et al (2019) Mechanosensitive meningeal nociception via Piezo channels: Implications for pulsatile pain in migraine? Neuropharmacology 149 113 PMID: 30768945

Jetta et al (2019) Shear stress-induced nuclear shrinkage through activation of Piezo1 channels in epithelial cells. J.Cell Sci. 132 jcs226076 PMID: 31076516

Cahalan et al (2015) Piezo1 links mechanical forces to red blood cell volume. Elife. 4 e07370 PMID: 26001274


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Keywords: Yoda 1, Yoda 1 supplier, Yoda1, Piezo1, calcium, channels, activators, sensitizers, sensitizes, Ca2+, currents, mechanotransduction, Piezo, Channels, 5586, Tocris Bioscience

38 Citations for Yoda 1

Citations are publications that use Tocris products. Selected citations for Yoda 1 include:

Ophir D et al (2021) Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids. Cell Stem Cell 28 1516-1532.e14 PMID: 33915079

Udo et al (2021) Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone Formation. J Bone Miner Res 36 369-384 PMID: 33180356

Adler L et al (2021) Mammary mechanobiology - investigating roles for mechanically activated ion channels in lactation and involution. J Cell Sci 134 PMID: 33262312

Ping et al (2021) TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection. Nat Commun 12 3519 PMID: 34112781

Yuri et al (2021) Selective Chemical Activation of Piezo1 in Leukemia Cell Membrane: Single Channel Analysis. Int J Mol Sci 22 PMID: 34360605

Chii Jou et al (2021) Cell fate coordinates mechano-osmotic forces in intestinal crypt formation. Nat Cell Biol 23 733-744 PMID: 34155381

Yu et al (2021) A role of PIEZO1 in iron metabolism in mice and humans. Cell 184 969-982.e13 PMID: 33571427

James et al (2022) Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels. Nat Commun 13 600 PMID: 35140203

James D et al (2022) Flow-Induced Secretion of Endothelial Heparanase Regulates Cardiac Lipoprotein Lipase and Changes Following Diabetes. J Am Heart Assoc 11 e027958 PMID: 36416172

Yan et al (2022) Mechanosensitive piezo1 calcium channel activates connexin 43 hemichannels through PI3K signaling pathway in bone. Cell Biosci 12 191 PMID: 36457052

Alla et al (2022) Store-Operated Ca2+ Entry Contributes to Piezo1-Induced Ca2+ Increase in Human Endometrial Stem Cells. Int J Mol Sci 23 PMID: 35409116

Steven R et al (2022) Piezo1-mediated stellate cell activation causes pressure-induced pancreatic fibrosis in mice. JCI Insight 7 PMID: 35451372

Rupalatha et al (2022) Mechanical Load and Piezo1 Channel Regulated Myosin II Activity in Mouse Lenses. Int J Mol Sci 23 PMID: 35563101

Katja M et al (2022) Microglial amyloid beta clearance is driven by PIEZO1 channels. J Neuroinflammation 19 147 PMID: 35706029

Ting et al (2022) Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis. Blood 140 171-183 PMID: 35443048

Ilona et al (2022) Mechanoreceptor Piezo1 Is Downregulated in Multiple Sclerosis Brain and Is Involved in the Maturation and Migration of Oligodendrocytes in vitro. Front Cell Neurosci 16 914985 PMID: 35722613

Stephanie W et al (2022) PIEZO1 mechanoreceptor activation reduces adipogenesis in perivascular adipose tissue preadipocytes. Front Endocrinol (Lausanne) 13 995499 PMID: 36120469

Willem-Jan et al (2022) A mechanical G2 checkpoint controls epithelial cell division through E-cadherin-mediated regulation of Wee1-Cdk1. Cell Rep 41 111475 PMID: 36223752

Yuan et al (2022) Inhibition of chemically and mechanically activated Piezo1 channels as a mechanism for ameliorating atherosclerosis with salvianolic acid B. Br J Pharmacol 179 3778-3814 PMID: 35194776

Yannick et al (2022) Piezo channels contribute to the regulation of myelination in Schwann cells. Glia 70 2276-2289 PMID: 35903933

Dela Paz (2018) Yoda1-induced phosphorylation of Akt and ERK1/2 does not require Piezo1 activation. Biochem Biophys Res Commun 497 220 PMID: 29428723

Tsuchiya (2018) Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nat Commun 9 2049 PMID: 29799007

Adler L et al (2020) Mammary mechanobiology: Investigating roles for mechanically-activated ion channels in lactation and involution. J Cell Sci PMID: 34005376

Qi et al (2020) Piezo1-Mediated Ca2+ Activities Regulate Brain Vascular Pathfinding during Development. Neuron 108 180-192.e5 PMID: 32827455

Stuart et al (2020) Inhibition of Piezo1 attenuates demyelination in the central nervous system. Glia 68 356-375 PMID: 31596529

Maria et al (2020) Piezo1 regulates calcium oscillations and cytokine release from astrocytes. Glia 68 145-160 PMID: 31433095

Bo et al (2020) Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-β-catenin. Elife 9 PMID: 32186512

Rodger A et al (2020) Piezo1 acts upstream of TRPV4 to induce pathological changes in endothelial cells due to shear stress. J Biol Chem 296 100171 PMID: 33298523

Jian et al (2020) Sphingomyelinase Disables Inactivation in Endogenous PIEZO1 Channels. Cell Rep 33 108225 PMID: 33027663

T Simon et al (2020) Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells. Elife 9 PMID: 32484440

Erin J et al (2020) Caenorhabditis elegans PIEZO channel coordinates multiple reproductive tissues to govern ovulation. Elife 9 PMID: 32490809

Stephen J et al (2020) TRPV4 channel opening mediates pressure-induced pancreatitis initiated by Piezo1 activation. J Clin Invest 130 2527-2541 PMID: 31999644

Yaganoglu et al (2023) Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi. Nat Commun 14 4352 PMID: 37468521

Cynthia A et al (2023) Matrix stiffness induces epithelial-to-mesenchymal transition via Piezo1-regulated calcium flux in prostate cancer cells. iScience 26 106275 PMID: 36950111

Ping et al (2023) Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells. Br J Pharmacol 180 1862-1877 PMID: 36740831

Romac et al (2018) Piezo1 is a mechanically activated ion channel and mediates pressure induced pancreatitis. Nat Commun 9 1715 PMID: 29712913

Ilkan et al (2017) Evidence for shear-mediated Ca2+ entry through mechanosensitive cation channels in human platelets and a megakaryocytic cell line. J Biol Chem 292 9204 PMID: 28416610

Chubinskiy-Nadezhdin et al (2019) Agonist-induced Piezo1 activation suppresses migration of transformed fibroblasts. Biochem Biophys Res Commun. 514 173 PMID: 31029419


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Reviews for Yoda 1

Average Rating: 5 (Based on 2 Reviews.)

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Stimulation of Piezo1 activity.
By Vladislav Chubinskiy-Nadezhdin on 08/16/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: 3T3B-SV40

Used in patch-clamp, cell migration assay (1-30 uM)

PMID: 31029419

A potent piezo1 receptor activator.
By Hyun Choi on 02/21/2018
Assay Type: Ex Vivo
Species: Rat

We used to stimulate piezo1 receptor at around 1-10microM