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Description: Piezo1 channel activator
Chemical Name: 2-[5-[[(2,6-Dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]pyrazine
Purity: ≥99% (HPLC)
Biological Activity for Yoda 1
Yoda 1 is a selective activator of mouse and human mechanosensitive channel piezo1. In HEK cells, Yoda 1 slows the inactivation phase of transient currents, sensitizes Piezo1 to activation by pressure, and partially activates channels in the absence of external pressure. In MDCK cells, Yoda 1 induces Ca2+-dependent chromatin hypercondensation and alters Ca2+-dependent myosin contractility, leading to nuclear shrinkage in cells. In red blood cells from sickle cell anemia (SCA) patients, Yoda 1 increases intracellular Ca2+ and phosphatidylserine exposure, which leads to KCa3.1 channel mediated Ca2+ influx and K+ and water efflux, causing shrinkage of the cells.
Compound Libraries for Yoda 1
Yoda 1 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for Yoda 1
| M. Wt | 355.27 |
| Formula | C13H8Cl2N4S2 |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 448947-81-7 |
| PubChem ID | 2746822 |
| InChI Key | BQNXBSYSQXSXPT-UHFFFAOYSA-N |
| Smiles | ClC1=C(CSC2=NN=C(C3=NC=CN=C3)S2)C(Cl)=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Yoda 1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.11 | 20 |
Preparing Stock Solutions for Yoda 1
The following data is based on the product molecular weight 355.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 14.07 mL | 70.37 mL | 140.74 mL |
| 1 mM | 2.81 mL | 14.07 mL | 28.15 mL |
| 2 mM | 1.41 mL | 7.04 mL | 14.07 mL |
| 10 mM | 0.28 mL | 1.41 mL | 2.81 mL |
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Product Datasheets for Yoda 1
References for Yoda 1
References are publications that support the biological activity of the product.
Syeda et al (2015) Chemical activation of the mechanotransduction channel Piezo1. ELife 4 e07369 PMID: 26001275
Mikhailov et al (2019) Mechanosensitive meningeal nociception via Piezo channels: Implications for pulsatile pain in migraine? Neuropharmacology 149 113 PMID: 30768945
Jetta et al (2019) Shear stress-induced nuclear shrinkage through activation of Piezo1 channels in epithelial cells. J.Cell Sci. 132 jcs226076 PMID: 31076516
Cahalan et al (2015) Piezo1 links mechanical forces to red blood cell volume. Elife. 4 e07370 PMID: 26001274
If you know of a relevant reference for Yoda 1, please let us know.
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Keywords: Yoda 1, Yoda 1 supplier, Yoda1, Piezo1, calcium, channels, activators, sensitizers, sensitizes, Ca2+, currents, mechanotransduction, Piezo, Channels, 5586, Tocris Bioscience
5 Citations for Yoda 1
Citations are publications that use Tocris products. Selected citations for Yoda 1 include:
Ilkan et al (2017) Evidence for shear-mediated Ca2+ entry through mechanosensitive cation channels in human platelets and a megakaryocytic cell line. J Biol Chem 292 9204 PMID: 28416610
Romac et al (2018) Piezo1 is a mechanically activated ion channel and mediates pressure induced pancreatitis. Nat Commun 9 1715 PMID: 29712913
Dela Paz (2018) Yoda1-induced phosphorylation of Akt and ERK1/2 does not require Piezo1 activation. Biochem Biophys Res Commun 497 220 PMID: 29428723
Tsuchiya (2018) Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nat Commun 9 2049 PMID: 29799007
Chubinskiy-Nadezhdin et al (2019) Agonist-induced Piezo1 activation suppresses migration of transformed fibroblasts. Biochem Biophys Res Commun. 514 173 PMID: 31029419
Do you know of a great paper that uses Yoda 1 from Tocris? Please let us know.
Reviews for Yoda 1
Average Rating: 5 (Based on 2 Reviews.)
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Stimulation of Piezo1 activity.
By
Vladislav Chubinskiy-Nadezhdin on 08/16/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: 3T3B-SV40
Used in patch-clamp, cell migration assay (1-30 uM)
A potent piezo1 receptor activator.
By
Hyun Choi on 02/21/2018
Assay Type: Ex Vivo
Species: Rat
We used to stimulate piezo1 receptor at around 1-10microM
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